小球藻对链脲佐菌素（STZ）诱导的糖尿病 Sprague-Dawley 大鼠氧化应激的调节作用。

Modulation of oxidative stress by Chlorella vulgaris in streptozotocin (STZ) induced diabetic Sprague-Dawley rats.

机构信息

Universiti Kebangsaan Malaysia Medical Center, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur, Malaysia.

出版信息

Adv Med Sci. 2010;55(2):281-8. doi: 10.2478/v10039-010-0046-z.

DOI:10.2478/v10039-010-0046-z

PMID:21147697

Abstract

PURPOSE

Chlorella vulgaris (CV), a fresh water alga has been reported to have hypoglycemic effects. However, antioxidant and anti-inflammatory effects of CV in diabetic animals have not been investigated to date. The aim of the present study was to investigate the role of CV in inflammation and oxidative damage in STZ-induced diabetic rats.

MATERIALS AND METHODS

Male Sprague-Dawley rats (300 - 400g) were divided into 4 groups: control, CV, STZ-induced diabetic rats, and STZ rats treated with CV (150mg/kg body wt). Blood samples were drawn from orbital sinus at 1 and 4 weeks for determination of oxidative cellular damage (DNA damage and lipid peroxidation [malondialdehyde, MDA]), inflammation (tumour necrosis factor alpha, TNF-α) and antioxidant status (catalase, CAT, and superoxide dismutase, SOD).

RESULTS

CV did not have any effects on glucose levels in diabetic rats, over the 4 weeks of treatment. However, it reduced significantly DNA damage and blood MDA levels in STZ-induced diabetic rats compared to the control group. Plasma levels of TNF-α however did not show any significant changes in STZ-induced diabetic rats fed with CV. Antioxidant enzyme SOD showed no significant changes in all groups but CAT activity was reduced in STZ-induced diabetic rats compared to the control.

CONCLUSIONS

CV did not have hypoglycaemic effect but it has a protective role in STZ-induced diabetic rats by reducing oxidative DNA damage, and lipid peroxidation.

摘要

目的

已报道淡水藻类小球藻（CV）具有降血糖作用。然而，迄今为止，尚未研究 CV 对糖尿病动物的抗氧化和抗炎作用。本研究旨在探讨 CV 在链脲佐菌素（STZ）诱导的糖尿病大鼠炎症和氧化损伤中的作用。

材料和方法

雄性 Sprague-Dawley 大鼠（300-400g）分为 4 组：对照组、CV 组、STZ 诱导的糖尿病大鼠组和 CV（150mg/kg 体重）治疗的 STZ 大鼠组。在 1 周和 4 周时从眶窦采血，用于测定氧化细胞损伤（DNA 损伤和脂质过氧化[丙二醛，MDA]）、炎症（肿瘤坏死因子-α，TNF-α）和抗氧化状态（过氧化氢酶，CAT 和超氧化物歧化酶，SOD）。

结果

在 4 周的治疗过程中，CV 对糖尿病大鼠的血糖水平没有任何影响。然而，与对照组相比，它显著降低了 STZ 诱导的糖尿病大鼠的 DNA 损伤和血液 MDA 水平。然而，给予 CV 的 STZ 诱导的糖尿病大鼠的血浆 TNF-α水平没有显示出任何显著变化。SOD 抗氧化酶在所有组中均无明显变化，但 CAT 活性在 STZ 诱导的糖尿病大鼠中较对照组降低。