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Quantifying translational mobility in neurons: comparison between current optical techniques.量化神经元中的平移运动性：当前光学技术之间的比较。
J Neurosci. 2010 Dec 8;30(49):16409-16. doi: 10.1523/JNEUROSCI.3063-10.2010.
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Optical imaging of neuronal activity in tissue labeled by retrograde transport of Calcium Green Dextran.通过钙绿葡聚糖逆行转运标记的组织中神经元活动的光学成像。
Brain Res Brain Res Protoc. 1997 May;1(2):157-64. doi: 10.1016/s1385-299x(96)00024-4.
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Functional photoacoustic microscopy of pH.pH 的功能光声显微镜成像。
J Biomed Opt. 2011 Oct;16(10):100503. doi: 10.1117/1.3644495.
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Optical determination of electrical properties of red blood cell and Ehrlich ascites tumor cell membranes with fluorescent dyes.
Soc Gen Physiol Ser. 1986;40:199-210.
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Optical visualization of carbon nanotubes-a unifying linkage between microscopic and spectroscopic characterization techniques.碳纳米管的光学可视化——微观和光谱表征技术之间的统一联系。
Small. 2010 Sep 20;6(18):1968-73. doi: 10.1002/smll.201001019.
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New dimension in nano-imaging: breaking through the diffraction limit with scanning near-field optical microscopy.纳米成像的新维度：利用扫描近场光学显微镜突破衍射极限。
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Plasmonic beaming and active control over fluorescent emission.等离子体激元聚束和对荧光发射的主动控制。
Nat Commun. 2011;2:283. doi: 10.1038/ncomms1286.
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Parallel and patterned optogenetic manipulation of neurons in the brain slice using a DMD-based projector.基于数字微镜器件的投影仪对脑片神经元进行并行和图案化光遗传学操控。
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Blue fluorescent amino acid for biological spectroscopy and microscopy.用于生物光谱学和显微镜的蓝色荧光氨基酸。
Proc Natl Acad Sci U S A. 2017 Jun 6;114(23):6005-6009. doi: 10.1073/pnas.1705586114. Epub 2017 May 22.

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Astroglial Glutamate Signaling and Uptake in the Hippocampus.海马体中的星形胶质细胞谷氨酸信号传导与摄取
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Ribeye protein is intrinsically dynamic but is stabilized in the context of the ribbon synapse.肋眼蛋白本质上是动态的，但在带状突触的情况下是稳定的。
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Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs).细胞的物理化学性质及其对内在无序蛋白质（IDPs）的影响。
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Multiple spatial and kinetic subpopulations of CaMKII in spines and dendrites as resolved by single-molecule tracking PALM.通过单分子追踪 PALM 解析出树突棘和树突中 CaMKII 的多个空间和动力学亚群。
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Munc18-1 protein molecules move between membrane molecular depots distinct from vesicle docking sites.Munc18-1 蛋白分子在不同于囊泡停泊位点的膜分子库之间移动。
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Direct measurement of diffusion in olfactory cilia using a modified FRAP approach.使用改良的 FRAP 方法直接测量嗅纤毛中的扩散。
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本文引用的文献

1
Probing cellular events, one quantum dot at a time.逐点探测细胞事件。
Nat Methods. 2010 Apr;7(4):275-85. doi: 10.1038/nmeth.1444. Epub 2010 Mar 30.
2
Analysis of mitochondrial dynamics and functions using imaging approaches.使用成像方法分析线粒体动力学和功能。
Curr Protoc Cell Biol. 2010 Mar;Chapter 4:Unit 4.25.1-21. doi: 10.1002/0471143030.cb0425s46.
3
Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy.通过受激发射突破衍射分辨率极限：受激发射损耗荧光显微镜技术
Opt Lett. 1994 Jun 1;19(11):780-2. doi: 10.1364/ol.19.000780.
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Beyond photobleaching, laser illumination unbinds fluorescent proteins.除了光漂白外，激光照射还会使荧光蛋白解离。
J Phys Chem B. 2009 Apr 16;113(15):5225-33. doi: 10.1021/jp8060152.
5
Measuring diffusion of lipid-like probes in artificial and natural membranes by raster image correlation spectroscopy (RICS): use of a commercial laser-scanning microscope with analog detection.通过光栅图像相关光谱法（RICS）测量脂质样探针在人工膜和天然膜中的扩散：使用具有模拟检测功能的商用激光扫描显微镜。
Langmuir. 2009 May 5;25(9):5209-18. doi: 10.1021/la8040538.
6
Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method.利用互相关光栅图像光谱法从激光扫描共聚焦图像序列中检测活细胞中的蛋白质复合物。
Biophys J. 2009 Jan;96(2):707-16. doi: 10.1016/j.bpj.2008.09.051.
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Direct observation of the nanoscale dynamics of membrane lipids in a living cell.活细胞中膜脂纳米级动力学的直接观察。
Nature. 2009 Feb 26;457(7233):1159-62. doi: 10.1038/nature07596. Epub 2008 Dec 21.
8
Spatial diffusivity and availability of intracellular calmodulin.细胞内钙调蛋白的空间扩散率与可用性
Biophys J. 2008 Dec 15;95(12):6002-15. doi: 10.1529/biophysj.108.138974. Epub 2008 Sep 26.
9
Macromolecular crowding and size effects on probe microviscosity.大分子拥挤效应及尺寸对探针微粘度的影响。
Biophys J. 2008 Dec;95(11):5362-73. doi: 10.1529/biophysj.108.131250. Epub 2008 Sep 12.
10
Structural plasticity with preserved topology in the postsynaptic protein network.突触后蛋白网络中具有保留拓扑结构的结构可塑性。
Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12587-92. doi: 10.1073/pnas.0711669105. Epub 2008 Aug 22.

Quantifying translational mobility in neurons: comparison between current optical techniques.

作者信息

Kim Sally A, Sanabria Hugo, Digman Michelle A, Gratton Enrico, Schwille Petra, Zipfel Warren R, Waxham M Neal

机构信息

Department of Neurobiology and Anatomy, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA.

出版信息

J Neurosci. 2010 Dec 8;30(49):16409-16. doi: 10.1523/JNEUROSCI.3063-10.2010.

DOI:10.1523/JNEUROSCI.3063-10.2010
PMID:21147979
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3003925/
Abstract
摘要