Linköping University, Department of Biomedical Engineering, S-581 85 Linköping, Sweden.
J Biomed Opt. 2010 Sep-Oct;15(5):057005. doi: 10.1117/1.3495719.
I use Monte Carlo simulations and phantom measurements to characterize a probe with adjacent optical fibres for diffuse reflectance spectroscopy during stereotactic surgery in the brain. Simulations and measurements have been fitted to a modified Beer-Lambert model for light transport in order to be able to quantify chromophore content based on clinically measured spectra in brain tissue. It was found that it is important to take the impact of the light absorption into account when calculating the apparent optical path length, lp, for the photons in order to get good estimates of the absorption coefficient, μa. The optical path length was found to be well fitted to the equation lp=a+b ln(Is)+c ln(μa)+d ln(Is)ln(μa), where Is is the reflected light intensity for scattering alone (i.e., zero absorption). Although coefficients a-d calculated in this study are specific to the probe used here, the general form of the equation should be applicable to similar probes.
我使用蒙特卡罗模拟和体模测量来描述一种带有相邻光纤的探头，用于在脑部立体定向手术中的漫反射光谱测量。模拟和测量结果符合改进的 Beer-Lambert 模型，用于光在组织中的传输，以便能够基于临床测量的脑组织光谱定量测定发色团含量。结果发现，在计算光子的表观光程 lp 时，必须考虑光吸收的影响，以便对吸收系数μa进行良好的估计。光程被发现与方程 lp=a+b ln(Is)+c ln(μa)+d ln(Is)ln(μa)很好地拟合，其中 Is 是仅散射的反射光强度（即零吸收）。尽管本研究中计算的系数 a-d 特定于此处使用的探头，但该方程的一般形式应适用于类似的探头。
