pH 的功能光声显微镜成像。
Functional photoacoustic microscopy of pH.
机构信息
Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130, USA.
出版信息
J Biomed Opt. 2011 Oct;16(10):100503. doi: 10.1117/1.3644495.
Abstract
pH is a tightly regulated indicator of metabolic activity. In mammalian systems, an imbalance of pH regulation may result from or result in serious illness. In this paper, we report photoacoustic microscopy (PAM) of a commercially available pH-sensitive fluorescent dye (SNARF-5F carboxylic acid) in tissue phantoms. We demonstrated that PAM is capable of pH imaging in absolute values at tissue depths of up to 2.0 mm, greater than possible with other forms of optical microscopy.
摘要
pH 值是代谢活动的一个严格调节指标。在哺乳动物系统中，pH 值调节的失衡可能是严重疾病的结果或起因。在本文中，我们报告了在组织体模中使用商业上可用的 pH 敏感荧光染料 (SNARF-5F 羧酸) 的光声显微镜 (PAM)。我们证明了 PAM 能够在组织深度达 2.0 毫米的范围内进行绝对值的 pH 成像，这比其他形式的光学显微镜更有优势。
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本文引用的文献
1
In vivo functional photoacoustic microscopy of cutaneous microvasculature in human skin.人体皮肤中皮肤微血管的活体功能光声显微镜成像。
J Biomed Opt. 2011 Feb;16(2):026004. doi: 10.1117/1.3536522.
2
In vivo photoacoustic microscopy of human cutaneous microvasculature and a nevus.人体皮肤微血管和痣的活体光声显微镜成像
J Biomed Opt. 2011 Jan-Feb;16(1):016015. doi: 10.1117/1.3528661.
3
Bimodal MR-PET agent for quantitative pH imaging.双模态 MR-PET 示踪剂用于定量 pH 成像。
Angew Chem Int Ed Engl. 2010 Mar 22;49(13):2382-4. doi: 10.1002/anie.201000075.
4
Multiscale photoacoustic microscopy and computed tomography.多尺度光声显微镜和计算机断层扫描。
Nat Photonics. 2009 Aug 29;3(9):503-509. doi: 10.1038/nphoton.2009.157.
5
Noninvasive, in vivo imaging of the mouse brain using photoacoustic microscopy.使用光声显微镜对小鼠大脑进行无创活体成像。
J Appl Phys. 2009 May 15;105(10):102027. doi: 10.1063/1.3116134. Epub 2009 May 19.
6
Noninvasive, in vivo imaging of blood-oxygenation dynamics within the mouse brain using photoacoustic microscopy.使用光声显微镜对小鼠脑内血液氧合动力学进行无创、活体成像。
J Biomed Opt. 2009 Mar-Apr;14(2):020502. doi: 10.1117/1.3095799.
7
Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate.使用超极化13C标记的碳酸氢盐对体内pH值进行磁共振成像。
Nature. 2008 Jun 12;453(7197):940-3. doi: 10.1038/nature07017. Epub 2008 May 28.
8
Ratiometric photoacoustic sensing of pH using a "sonophore".使用“声敏剂”对pH进行比率光声传感。
Analyst. 2008 Jun;133(6):747-9. doi: 10.1039/b800116b. Epub 2008 Mar 6.
9
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Nat Biotechnol. 2006 Jul;24(7):848-51. doi: 10.1038/nbt1220. Epub 2006 Jun 25.
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