Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130, USA.
J Biomed Opt. 2011 Oct;16(10):100503. doi: 10.1117/1.3644495.
pH is a tightly regulated indicator of metabolic activity. In mammalian systems, an imbalance of pH regulation may result from or result in serious illness. In this paper, we report photoacoustic microscopy (PAM) of a commercially available pH-sensitive fluorescent dye (SNARF-5F carboxylic acid) in tissue phantoms. We demonstrated that PAM is capable of pH imaging in absolute values at tissue depths of up to 2.0 mm, greater than possible with other forms of optical microscopy.
pH 值是代谢活动的一个严格调节指标。在哺乳动物系统中，pH 值调节的失衡可能是严重疾病的结果或起因。在本文中，我们报告了在组织体模中使用商业上可用的 pH 敏感荧光染料 (SNARF-5F 羧酸) 的光声显微镜 (PAM)。我们证明了 PAM 能够在组织深度达 2.0 毫米的范围内进行绝对值的 pH 成像，这比其他形式的光学显微镜更有优势。
