Departamento de Producción Agraria, Universidad Pública de Navarra, E-31006 Pamplona, España.
Mycologia. 2004 Jan-Feb;96(1):41-51.
Fluorescence microscopy was used to describe the distribution of nuclei and the organization of the microtubule network in hyphae of Pleurotus ostreatus. Dikaryotic hyphae of P. ostreatus N001 grow by tip extension with two closely spaced nuclei moving slowly forward with the growing hyphal tip. During vegetative growth of the hyphae, cytoplasmic microtubules are found as long filaments oriented longitudinally within fungal hyphae. When the apical cell reaches a length of approximately 150 μm, the two nuclei divide synchronously. Mitosis occurs in association with clamp connection formation, with one of the nuclei dividing in the hook of the developing clamp connection and the other in the main hypha. After mitosis, two daughter nuclei move forward to approximately the center of the apical cell, while the other two move backward to a central position in the subapical cell. Two septa are formed, one in the clamp and the other across the main axis of the hypha to delimit the apical cell. The use of fluorescence microscopy made it possible to examine the changes in the cytoplasmic microtubules, the configuration of the mitotic apparatus, the site of septation and the post-mitotic nuclear migrations during conjugate division in P. ostreatus dikaryotic hyphae.
荧光显微镜用于描述糙皮侧耳（Pleurotus ostreatus）菌丝中细胞核的分布和微管网络的组织。糙皮侧耳 N001 的双核菌丝通过尖端延伸生长，两个紧密间隔的细胞核缓慢地随生长的菌丝尖端向前移动。在菌丝的营养生长过程中，细胞质微管被发现为长丝，在真菌菌丝内纵向定向。当顶端细胞达到大约 150μm 的长度时，两个细胞核同步分裂。有丝分裂与夹点连接的形成有关，其中一个细胞核在发育夹点连接的钩中分裂，另一个在主菌丝中分裂。有丝分裂后，两个子核向前移动到大约顶端细胞的中心，而另外两个核向后移动到亚顶端细胞的中心位置。形成两个隔膜，一个在夹点中，另一个穿过菌丝的主轴，以限定顶端细胞。荧光显微镜的使用使得有可能检查糙皮侧耳双核菌丝中细胞质微管的变化、有丝分裂装置的构象、隔膜的位置以及有丝分裂后核的迁移，这些都是在双核菌丝的配合分裂过程中发生的。
