Friedrich Schiller University, Institute of Microbiology, Neugasse 25, 07743 Jena, Germany.
Friedrich Schiller University, Institute of Microbiology, Neugasse 25, 07743 Jena, Germany. Electronic address: http://www.mikrobio.uni-jena.de.
Fungal Genet Biol. 2018 Feb;111:85-91. doi: 10.1016/j.fgb.2017.11.001. Epub 2017 Nov 10.
Basidiomycetes feature a prolonged dikaryotic life stage. A dispute over open versus closed mitosis could be solved using in vivo fluorescence videomicroscopy of histone 2B::EGFP and Lifeact labeled Schizophyllum commune. It revealed nuclei to condense to approximately one fifth in diameter during mitotic prophase. In addition, the specifics of clamp cell formation typical of many basidiomycetes included an actin network at the future site of nuclear division, which allowed for cessation of nuclear movement and re-localization of one nucleus towards the emerging clamp cell, while the other divided along the hyphal axis. Subsequent fusion of the clamp cell to form the clamp connection restored the close association of the two nuclei in a very fast process after clamp fusion. Septation was preceded by actin patches and vesicles involved in formation of the actin ring.
担子菌门具有一个延长的双核生命阶段。使用活体荧光显微镜观察组蛋白 2B::EGFP 和 Lifeact 标记的裂褶菌，可以解决有丝分裂是开放还是闭合的争议。结果表明，在有丝分裂前期，细胞核直径大约缩小到原来的五分之一。此外，许多担子菌特有的夹状细胞形成的具体过程包括在未来核分裂部位的肌动蛋白网络，该网络允许核停止运动，并使一个核重新定位到即将形成的夹状细胞，而另一个核沿着菌丝轴分裂。随后，夹状细胞融合形成夹状连接，在夹状融合后非常快速的过程中恢复了两个核的紧密联系。隔膜形成之前，有肌动蛋白斑点和参与肌动蛋白环形成的小泡。
