Department of Botany, Damietta Faculty of Science, Mansoura University, New Damietta, Damietta Province, Egypt. P.O. Box 34517.
Mycologia. 2004 Nov-Dec;96(6):1183-9.
Thirty-two fungal species were isolated from a polluted watercourse near the Talkha fertilizer plant, Mansoura Province, Egypt. Aspergillus niger, A. flavus, Cunninghamella echinulata and Trichoderma koningii were isolated frequently. On the basis of its frequency, Cunninghamella echinulata was chosen for biosorption studies. Free and immobilized biomass of C. echinulata sequestered ions in this decreasing sequence is: Pb >Cu >Zn. The effects of biomass concentration, pH and time of contact were investigated. The level of ion uptake rose with increasing biomass. The maximum uptake for lead (45 mg/g), copper (20 mg/g) and zinc (18.8 mg/g) occurred at 200 mg/L biomass. The uptake rose with increasing pH up to 4 in the case of Pb and 5 in the case of Cu and Zn. Maximum uptake for all metals was achieved after 15 min. Ion uptake followed the Langmuir adsorption model, permitting the calculation of maximum uptake and affinity coefficients. Treatment of C. echinulata biomass with NaOH improved biosorbent capacity, as did immobilization with alginate. Immobilized biomass could be regenerated readily by treatment with dilute HCl. The biomass-alginate complex efficiently removed Pb, Zn and Cu from polluted water samples. Therefore,Cunninghamella echinulata could be employed either in free or immobilized form as a biosorbent of metal ions in waste water.
从埃及曼苏拉省 Talkha 肥料厂附近受污染的水道中分离出 32 种真菌。黑曲霉、黄曲霉、卷枝毛霉和康宁木霉经常被分离出来。基于其频率，选择卷枝毛霉进行生物吸附研究。卷枝毛霉的游离和固定化生物量对离子的螯合顺序为：Pb>Cu>Zn。研究了生物量浓度、pH 值和接触时间的影响。离子摄取水平随生物量的增加而增加。最大铅（45mg/g）、铜（20mg/g）和锌（18.8mg/g）的摄取量发生在 200mg/L 生物量时。在 Pb 的情况下，pH 值升高到 4，在 Cu 和 Zn 的情况下，pH 值升高到 5，吸附量增加。所有金属的最大摄取量在 15 分钟后达到。离子吸附遵循 Langmuir 吸附模型，允许计算最大吸附量和亲和系数。用 NaOH 处理卷枝毛霉生物质提高了生物吸附剂的容量，用藻酸盐固定化也提高了容量。用稀 HCl 处理可以很容易地再生固定化生物量。生物量-藻酸盐复合物可有效去除受污染水样中的 Pb、Zn 和 Cu。因此，卷枝毛霉可以以游离或固定化的形式作为废水中金属离子的生物吸附剂。
