Department of Biological Sciences, Life Sciences Building, Room 124, University of South Alabama, 307 University Blvd., Mobile, Alabama 36688-0002.
Mycologia. 2003 Nov-Dec;95(6):998-1003. doi: 10.1080/15572536.2004.11833015.
We examined the distribution of hyphae of the trichomycete fungus Smittium culisetae (Harpellales: Legeriomycetaceae) in the hindgut of a larval black fly (Simulium vittatum, cytospecies IS-7) by analyzing its prevalence and relative abundance. Hyphal prevalence was highest in the posterior colon (93.1%) and rectum (86.3%), with low prevalence (12.0%) in the anterior colon. Relative abundance of hyphae was highest in the posterior colon, followed by the rectum; relative abundance of hyphae in the anterior colon was lower. Hyphae of S. culisetae were not observed in the pylorus. We used a novel method of quantifying the relative abundance of S. culisetae in the host hindgut. The hindgut was observed with an ocular grid, and abundance was expressed as the ratio of grids occupied by hyphae to the number of grids occupied by hindgut.
我们通过分析其流行率和相对丰度，研究了 Trichomycete 真菌 Smittium culisetae（Harpellales：Legeriomycetaceae）在幼虫黑蝇（Simulium vittatum，cytospecies IS-7）后肠中的菌丝分布。菌丝的流行率在后肠（93.1%）和直肠（86.3%）中最高，在前肠中较低（12.0%）。菌丝的相对丰度在后肠中最高，其次是直肠；前肠中的菌丝相对丰度较低。幽门中未观察到 S. culisetae 的菌丝。我们使用了一种新的方法来量化宿主后肠中 S. culisetae 的相对丰度。用目镜网格观察后肠，并将丰度表示为菌丝占据的网格数与后肠占据的网格数之比。
