Embrapa Agrobiologia, Caixa Postal 74505, CEP 23851-970, Seropédica, RJ, Brazil Department of Plant Microorganism Interaction.
Mycologia. 2003 Nov-Dec;95(6):1004-12.
Mycelium development and architecture and spore production were studied in Scutellospora reticulata from single-spore isolates grown with Ri T-DNA transformed carrot root-organ culture in monoxenic system. Culture establishment, anastomosis occurrence and auxiliary cell development also were examined. Seventy percent of the pregerminated disinfected spores colonized the transformed carrot roots. After 8 mo, the average spore production was 56 (24-130) per 30 cm(3) of medium. Of the spores produced, 75% germinated and produced new generations in monoxenic culture. The mycelium network was formed by thick light-brown hyphae, which exhibit two major architecture patterns related to either root colonization or resource exploitation, and lower-order hyphae, bearing auxiliary cells, branched absorbing structures (BAS), hyphal swellings (HS) and forming anastomoses. BAS were formed abundantly in extramatrical mycelium and frequently had HS resembling vesicles, a feature not previously reported in the Gigasporaceae, to the best of our knowledge. Few anastomosis were observed within the mycelium and most often corresponded to a healing mechanism that form hypha bridges to reconnect broken hyphae or overcoming obstructed areas within a hypha. Numerous auxiliary cells were produced during culture development and their role was inferred.
在单养系统中，用 Ri T-DNA 转化的胡萝卜根器官培养物培养的单孢分离物中研究了 Scutellospora reticulata 的菌丝体发育和结构以及孢子产生。还检查了培养物的建立、吻合发生和辅助细胞的发育。70%的预萌发消毒孢子定殖于转化的胡萝卜根中。8 个月后，平均每 30cm3 培养基的孢子产量为 56（24-130）个。在单养培养中，产生的孢子中有 75%发芽并产生新世代。菌丝体网络由厚的浅棕色菌丝组成，其表现出与根定殖或资源利用相关的两种主要结构模式，以及较低阶的菌丝，带有辅助细胞、分枝吸收结构（BAS）、菌丝膨大和形成吻合。BAS 在基质外的菌丝体中大量形成，并经常具有类似于囊泡的 HS，据我们所知，这在 Gigasporeaceae 中尚未报道过。在菌丝体中观察到的吻合很少，通常对应于一种愈合机制，该机制形成菌丝桥以重新连接断裂的菌丝或克服菌丝内的阻塞区域。在培养物发育过程中产生了大量的辅助细胞，并推断了它们的作用。
