Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar (ICIDCA), Vía Blanca 804, P.O. Box 4026, La Habana, Cuba.
Mycologia. 2003 Nov-Dec;95(6):1013-20. doi: 10.1080/15572536.2004.11833017.
Four laccase isozymes (LCC1, LCC2, LCC3 and LCC4) synthesized by Pleurotus ostreatus strain V-184 were purified and characterized. LCC1 and LCC2 have molecular masses of about 60 and 65 kDa and exhibited the same pI value (3.0). Their N termini were sequenced, revealing the same amino acid sequence and homology with laccases from other microorganisms. Laccases LCC3 and LCC4 were characterized by SDS-PAGE, estimating their molecular masses around 80 and 82 kDa, respectively. By native isoelectrofocusing, their pI values were 4.7 and 4.5, respectively. When staining with ABTS and guaiacol in native polyacrilamide gels, different specificities were observed for LCC1/LCC2 and LCC3/LCC4 isozymes.
四种漆酶同工酶（LCC1、LCC2、LCC3 和 LCC4）由糙皮侧耳 V-184 菌株合成并进行了纯化和特性分析。LCC1 和 LCC2 的分子量约为 60 和 65 kDa，等电点（pI）值相同（3.0）。它们的 N 端序列被测序，显示与来自其他微生物的漆酶具有相同的氨基酸序列和同源性。同工酶 LCC3 和 LCC4 通过 SDS-PAGE 进行了表征，估计它们的分子量分别约为 80 和 82 kDa。通过天然等电聚焦，它们的 pI 值分别为 4.7 和 4.5。在用 ABTS 和愈创木酚在天然聚丙烯酰胺凝胶中染色时，观察到 LCC1/LCC2 和 LCC3/LCC4 同工酶具有不同的特异性。
