• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

果蝇同源异型盒复合体 bithorax 中边界与 Abd-B 启动子上游区的选择性相互作用及 dCTCF 在这一过程中的作用。

Selective interactions of boundaries with upstream region of Abd-B promoter in Drosophila bithorax complex and role of dCTCF in this process.

机构信息

Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St, Moscow 119334, Russia.

出版信息

Nucleic Acids Res. 2011 Apr;39(8):3042-52. doi: 10.1093/nar/gkq1248. Epub 2010 Dec 10.

DOI:10.1093/nar/gkq1248
PMID:21149269
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3082887/
Abstract

Expression of the genes Ubx, abd-A, and Abd-B of the bithorax complex depends on its cis-regulatory region, which is divided into discrete functional domains (iab). Boundary/insulator elements, named Mcp, Fab-6, Fab-7 and Fab-8 (PTS/F8), have been identified at the borders of the iab domains. Recently, binding sites for a Drosophila homolog of the vertebrate insulator protein CTCF have been identified in Mcp, Fab-6 and Fab-8 and also in several regions that correspond to predicted boundaries, Fab-3 and Fab-4 in particular. Taking into account the inability of the yeast GAL4 activator to stimulate the white promoter when the activator and the promoter are separated by a 5-kb yellow gene, we have tested functional interactions between the boundaries. The results show that all dCTCF-containing boundaries interact with each other. However, inactivation of dCTCF binding sites in Mcp, Fab-6 and PTS/F8 only partially reduces their ability to interact, suggesting the presence of additional protein(s) supporting distant interactions between the boundaries. Interestingly, only Fab-6, Fab-7 (which contains no dCTCF binding sites) and PTS/F8 interact with the upstream region of the Abd-B promoter. Thus, the boundaries might be involved in supporting the specific interactions between iab enhancers and promoters of the bithorax complex.

摘要

双胸复合体基因 Ubx、abd-A 和 Abd-B 的表达取决于其顺式调控区，该区域被分为离散的功能域（iab）。边界/绝缘子元件，命名为 Mcp、Fab-6、Fab-7 和 Fab-8（PTS/F8），已在 iab 域的边界处被鉴定。最近，在 Mcp、Fab-6 和 Fab-8 以及对应于预测边界的几个区域（特别是 Fab-3 和 Fab-4）中，已经鉴定出与脊椎动物绝缘子蛋白 CTCF 的果蝇同源物的结合位点。考虑到酵母 GAL4 激活剂不能在激活剂和启动子之间被 5kb 的黄色基因隔开时刺激白色启动子时的无能，我们已经测试了边界之间的功能相互作用。结果表明，所有含有 dCTCF 的边界都相互作用。然而，Mcp、Fab-6 和 PTS/F8 中的 dCTCF 结合位点失活仅部分降低了它们相互作用的能力，表明存在额外的蛋白质（s）支持边界之间的远距离相互作用。有趣的是，只有 Fab-6、Fab-7（不含 dCTCF 结合位点）和 PTS/F8 与 Abd-B 启动子的上游区域相互作用。因此，边界可能参与支持iab 增强子和双胸复合体启动子之间的特异性相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/fdf9b162e096/gkq1248f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/789c19d3a673/gkq1248f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/19f08c4c9560/gkq1248f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/25fc23205709/gkq1248f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/78bfe1f59e85/gkq1248f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/e6645a4434bc/gkq1248f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/304da63aa747/gkq1248f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/fdf9b162e096/gkq1248f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/789c19d3a673/gkq1248f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/19f08c4c9560/gkq1248f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/25fc23205709/gkq1248f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/78bfe1f59e85/gkq1248f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/e6645a4434bc/gkq1248f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/304da63aa747/gkq1248f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8271/3082887/fdf9b162e096/gkq1248f7.jpg

相似文献

1
Selective interactions of boundaries with upstream region of Abd-B promoter in Drosophila bithorax complex and role of dCTCF in this process.果蝇同源异型盒复合体 bithorax 中边界与 Abd-B 启动子上游区的选择性相互作用及 dCTCF 在这一过程中的作用。
Nucleic Acids Res. 2011 Apr;39(8):3042-52. doi: 10.1093/nar/gkq1248. Epub 2010 Dec 10.
2
Functional interaction between the Fab-7 and Fab-8 boundaries and the upstream promoter region in the Drosophila Abd-B gene.果蝇Abd - B基因中Fab - 7和Fab - 8边界与上游启动子区域之间的功能相互作用。
Mol Cell Biol. 2008 Jun;28(12):4188-95. doi: 10.1128/MCB.00229-08. Epub 2008 Apr 21.
3
Fragments of the Fab-3 and Fab-4 Boundaries of the Drosophila melanogaster Bithorax Complex That Include CTCF Sites Are not Effective Insulators.果蝇体节发育基因复合体的 Fab-3 和 Fab-4 边界片段，包含 CTCF 结合位点，但不具有有效的绝缘子活性。
Dokl Biochem Biophys. 2022 Feb;502(1):21-24. doi: 10.1134/S1607672922010069. Epub 2022 Mar 11.
4
Functional Dissection of the Blocking and Bypass Activities of the Fab-8 Boundary in the Drosophila Bithorax Complex.果蝇双胸复合体中Fab-8边界阻断和旁路活性的功能剖析
PLoS Genet. 2016 Jul 18;12(7):e1006188. doi: 10.1371/journal.pgen.1006188. eCollection 2016 Jul.
5
Architectural protein Pita cooperates with dCTCF in organization of functional boundaries in Bithorax complex.结构蛋白Pita与dCTCF在双胸复合体功能边界的组织中相互协作。
Development. 2017 Jul 15;144(14):2663-2672. doi: 10.1242/dev.149815. Epub 2017 Jun 15.
6
Boundaries mediate long-distance interactions between enhancers and promoters in the Drosophila Bithorax complex.边界介导果蝇同源异型盒复合体中增强子和启动子之间的远程相互作用。
PLoS Genet. 2018 Dec 12;14(12):e1007702. doi: 10.1371/journal.pgen.1007702. eCollection 2018 Dec.
7
Complete reconstitution of bypass and blocking functions in a minimal artificial insulator from complex.从复杂体系中重建最小人工绝缘子的旁路和阻断功能。
Proc Natl Acad Sci U S A. 2019 Jul 2;116(27):13462-13467. doi: 10.1073/pnas.1907190116. Epub 2019 Jun 17.
8
Transvection in the Drosophila Abd-B domain: extensive upstream sequences are involved in anchoring distant cis-regulatory regions to the promoter.果蝇Abd - B结构域中的转位作用：广泛的上游序列参与将远距离顺式调控区域锚定到启动子上。
Genetics. 1998 Jun;149(2):1031-50. doi: 10.1093/genetics/149.2.1031.
9
CTCF genomic binding sites in Drosophila and the organisation of the bithorax complex.果蝇中CTCF的基因组结合位点与双胸复合体的组织
PLoS Genet. 2007 Jul;3(7):e112. doi: 10.1371/journal.pgen.0030112.
10
[Study of the enhancer-blocking activities of new boundaries in the bithorax complex of Drosophila melanogaster].[黑腹果蝇双胸复合体新边界增强子阻断活性的研究]
Genetika. 2011 Sep;47(9):1184-9.

引用本文的文献

1
Chromosome structure in is determined by boundary pairing not loop extrusion.染色质结构由边界配对决定，而不是环挤出。
Elife. 2024 Aug 7;13:RP94070. doi: 10.7554/eLife.94070.
2
Stem-loop and circle-loop TADs generated by directional pairing of boundary elements have distinct physical and regulatory properties.由边界元件定向配对产生的茎环和环loop TADs 具有不同的物理和调控性质。
Elife. 2024 Aug 7;13:RP94114. doi: 10.7554/eLife.94114.
3
The N-terminal dimerization domains of human and Drosophila CTCF have similar functionality.

本文引用的文献

1
The bithorax complex of Drosophila an exceptional Hox cluster.果蝇的双胸复合体——一个特殊的同源异型基因簇。
Curr Top Dev Biol. 2009;88:1-33. doi: 10.1016/S0070-2153(09)88001-0.
2
Genome wide ChIP-chip analyses reveal important roles for CTCF in Drosophila genome organization.全基因组芯片分析揭示了CTCF在果蝇基因组组织中的重要作用。
Dev Biol. 2009 Apr 15;328(2):518-28. doi: 10.1016/j.ydbio.2008.12.039. Epub 2009 Jan 8.
3
Boundary swapping in the Drosophila Bithorax complex.果蝇双胸复合体中的边界交换
人源和果蝇 CTCF 的 N 端二聚化结构域具有相似的功能。
Epigenetics Chromatin. 2024 Apr 1;17(1):9. doi: 10.1186/s13072-024-00534-w.
4
CRISPR/Cas9 and FLP-FRT mediated regulatory dissection of the BX-C of Drosophila melanogaster.CRISPR/Cas9 和 FLP-FRT 介导的果蝇 BX-C 的调控细分。
Chromosome Res. 2023 Jan 31;31(1):7. doi: 10.1007/s10577-023-09716-w.
5
The Variable CTCF Site from Drosophila melanogaster Ubx Gene is Redundant and Has no Insulator Activity.果蝇 Ubx 基因的可变 CTCF 结合位点是冗余的，没有绝缘子活性。
Dokl Biochem Biophys. 2022 Aug;505(1):173-175. doi: 10.1134/S1607672922040044. Epub 2022 Aug 29.
6
Homeotic Genes: Clustering, Modularity, and Diversity.同源异型基因：聚类、模块化与多样性
Front Cell Dev Biol. 2021 Aug 11;9:718308. doi: 10.3389/fcell.2021.718308. eCollection 2021.
7
Boundaries potentiate polycomb response element-mediated silencing.边界增强多梳反应元件介导的沉默。
BMC Biol. 2021 Jun 2;19(1):113. doi: 10.1186/s12915-021-01047-8.
8
Mapping of functional elements of the Fab-6 boundary involved in the regulation of the Abd-B hox gene in Drosophila melanogaster.在果蝇中，参与 Abd-B hox 基因调控的 Fab-6 边界功能元件的映射。
Sci Rep. 2021 Feb 18;11(1):4156. doi: 10.1038/s41598-021-83734-8.
9
The Functions and Mechanisms of Action of Insulators in the Genomes of Higher Eukaryotes.高等真核生物基因组中绝缘子的功能及作用机制
Acta Naturae. 2020 Oct-Dec;12(4):15-33. doi: 10.32607/actanaturae.11144.
10
Mechanisms of Enhancer-Promoter Interactions in Higher Eukaryotes.高等真核生物中增强子-启动子相互作用的机制。
Int J Mol Sci. 2021 Jan 12;22(2):671. doi: 10.3390/ijms22020671.
Development. 2008 Dec;135(24):3983-7. doi: 10.1242/dev.025700. Epub 2008 Nov 5.
4
Characterization of new regulatory elements within the Drosophila bithorax complex.果蝇双胸复合体中新调控元件的特征分析。
Nucleic Acids Res. 2008 Dec;36(21):6926-33. doi: 10.1093/nar/gkn818. Epub 2008 Oct 31.
5
The abdominal-B promoter tethering element mediates promoter-enhancer specificity at the Drosophila bithorax complex.腹部B启动子拴系元件在果蝇双胸复合体中介导启动子-增强子特异性。
Fly (Austin). 2007 Nov-Dec;1(6):337-9. doi: 10.4161/fly.5607.
6
Functional interaction between the Fab-7 and Fab-8 boundaries and the upstream promoter region in the Drosophila Abd-B gene.果蝇Abd - B基因中Fab - 7和Fab - 8边界与上游启动子区域之间的功能相互作用。
Mol Cell Biol. 2008 Jun;28(12):4188-95. doi: 10.1128/MCB.00229-08. Epub 2008 Apr 21.
7
Coordinated control of dCTCF and gypsy chromatin insulators in Drosophila.果蝇中dCTCF与gypsy染色质绝缘子的协同调控
Mol Cell. 2007 Dec 14;28(5):761-72. doi: 10.1016/j.molcel.2007.09.024.
8
A novel promoter-tethering element regulates enhancer-driven gene expression at the bithorax complex in the Drosophila embryo.一种新型的启动子连接元件在果蝇胚胎的双胸复合体中调节增强子驱动的基因表达。
Development. 2008 Jan;135(1):123-31. doi: 10.1242/dev.010744. Epub 2007 Nov 28.
9
A stage-specific factor confers Fab-7 boundary activity during early embryogenesis in Drosophila.一种阶段特异性因子在果蝇早期胚胎发育过程中赋予Fab-7边界活性。
Mol Cell Biol. 2008 Feb;28(3):1047-60. doi: 10.1128/MCB.01622-07. Epub 2007 Nov 26.
10
We gather together: insulators and genome organization.我们齐聚一堂：绝缘子与基因组组织。
Curr Opin Genet Dev. 2007 Oct;17(5):400-7. doi: 10.1016/j.gde.2007.08.005. Epub 2007 Oct 24.