Department of Neurobiology, Physiology, and Behavior, University of California, Davis, Davis, CA 95616, USA.
J Gen Physiol. 2011 Jan;137(1):3-16. doi: 10.1085/jgp.201010555. Epub 2010 Dec 13.
Voltage-gated ion channels underlie electrical activity of neurons and are dynamically regulated by diverse cell signaling pathways that alter their phosphorylation state. Recent global mass spectrometric-based analyses of the mouse brain phosphoproteome have yielded a treasure trove of new data as to the extent and nature of phosphorylation of numerous ion channel principal or α subunits in mammalian brain. Here we compile and review data on 347 phosphorylation sites (261 unique) on 42 different voltage-gated ion channel α subunits that were identified in these recent studies. Researchers in the ion channel field can now begin to explore the role of these novel in vivo phosphorylation sites in the dynamic regulation of the localization, activity, and expression of brain ion channels through multisite phosphorylation of their principal subunits.
电压门控离子通道是神经元电活动的基础，其活性可被多种细胞信号通路动态调节，这些通路通过改变其磷酸化状态来调节离子通道的功能。最近基于全局质谱分析的小鼠脑磷酸化蛋白质组学研究，为哺乳动物脑内许多离子通道主要或α亚基的磷酸化程度和性质提供了大量新的数据。在此，我们编译并综述了最近的研究中在 42 种不同的电压门控离子通道α亚基上发现的 347 个磷酸化位点（261 个独特）的数据。离子通道领域的研究人员现在可以开始通过其主要亚基的多位点磷酸化，来探索这些新的体内磷酸化位点在脑离子通道的定位、活性和表达的动态调节中的作用。
