Hültner L, Druez C, Moeller J, Uyttenhove C, Schmitt E, Rüde E, Dörmer P, Van Snick J
GSF-Institut für Experimentelle Hämatologie, Munich, FRG.
Eur J Immunol. 1990 Jun;20(6):1413-6. doi: 10.1002/eji.1830200632.
We have previously shown that certain bone marrow-derived mast cell (BMMC) lines proliferate in response to a mast cell growth-enhancing activity (MEA) that is distinct from interleukin (IL) 3 and IL 4. Here we provide evidence that MEA is identical with the recently cloned mouse T cell growth factor P40. The evidence is as follows: (a) recombinant P40 displayed all the biological activities ascribed to MEA: it supported the growth of MEA-sensitive BMMC lines, it induced IL 6 secretion by these cells, and it enhanced survival of primary mast cell cultures; (b) highly purified MEA stimulated the growth of P40-dependent cell lines; (c) a rabbit monospecific antiserum directed against P40 specifically inhibited the action of MEA on BMMC; (d) specific binding sites for P40 were detected on BMMC and (e) MEA competed with P40 for binding to P40-dependent T cells, indicating that the two molecules interact with the same receptor. These observations further extend the range of biological activities ascribed to P40 and warrant its proposed designation as IL9.
我们先前已表明，某些骨髓来源的肥大细胞（BMMC）系会对一种不同于白细胞介素（IL）-3和IL-4的肥大细胞生长增强活性（MEA）作出增殖反应。在此我们提供证据表明MEA与最近克隆的小鼠T细胞生长因子P40相同。证据如下：（a）重组P40展现出了归因于MEA的所有生物学活性：它支持对MEA敏感的BMMC系的生长，诱导这些细胞分泌IL-6，并提高原代肥大细胞培养物的存活率；（b）高度纯化的MEA刺激了依赖P40的细胞系的生长；（c）针对P40的兔单特异性抗血清特异性抑制了MEA对BMMC的作用；（d）在BMMC上检测到了P40的特异性结合位点；以及（e）MEA与P40竞争结合依赖P40的T细胞，表明这两种分子与同一受体相互作用。这些观察结果进一步扩展了归因于P40的生物学活性范围，并证明将其命名为IL-9是合理的。
