Neuroscience Institute, Lithuanian University of Health Sciences, Medical Academy, Eiveniu St. 2, Kaunas, Lithuania LT-50161.
Department of Ophthalmology, Lithuanian University of Health Sciences, Medical Academy, Eiveniu St. 2, Kaunas, Lithuania LT-50161.
Mediators Inflamm. 2021 Apr 12;2021:6622934. doi: 10.1155/2021/6622934. eCollection 2021.
Considering the immunological impairment in age-related macular degeneration (AMD), we aimed to determine the associations of rs1859430, rs2069870, rs11741137, rs2069885, and rs2069884 and rs1800871, rs1800872, and rs1800896 polymorphisms and their haplotypes, as well as the serum levels of IL-9 and IL-10 with AMD. 1209 participants were enrolled in our study. SNPs were genotyped using TaqMan SNP genotyping assays by real-time PCR method. IL-9 and IL-10 serum levels were evaluated using ELISA kits. Our study results have shown that haplotypes A-G-C-G-G and G-A-T-A-T of SNPs are associated with the decreased odds of early AMD occurrence ( = 0.035 and = 0.015, respectively). A set of rare haplotypes was associated with the decreased odds of exudative AMD occurrence ( = 0.033). Also, IL-10 serum levels were lower in exudative AMD than in controls ( = 0.049), patients with early AMD ( = 0.017), and atrophic AMD ( = 0.008). Furthermore, exudative AMD patients with rs1800896 CT and TT genotypes had lower IL-10 serum concentrations than those with wild-type (CC) genotype ( = 0.048). In conclusion, our study suggests that IL-10 serum levels can be associated with a minor allele at rs1800896 and exudative AMD. The haplotypes of IL-9 SNPs were also associated with the decreased odds of early and exudative AMD.
考虑到年龄相关性黄斑变性（AMD）中的免疫损伤，我们旨在确定 rs1859430、rs2069870、rs11741137、rs2069885 和 rs2069884 以及 rs1800871、rs1800872 和 rs1800896 多态性及其单倍型与 AMD 的关联，以及 IL-9 和 IL-10 的血清水平。我们的研究纳入了 1209 名参与者。使用 TaqMan SNP 基因分型测定法通过实时 PCR 方法对 SNPs 进行基因分型。使用 ELISA 试剂盒评估 IL-9 和 IL-10 的血清水平。我们的研究结果表明，SNP 的单倍型 A-G-C-G-G 和 G-A-T-A-T 与早期 AMD 发生的几率降低相关（ = 0.035 和 = 0.015）。一组罕见的单倍型与渗出性 AMD 发生的几率降低相关（ = 0.033）。此外，与对照组相比，渗出性 AMD 患者的 IL-10 血清水平较低（ = 0.049），与早期 AMD 患者（ = 0.017）和萎缩性 AMD 患者（ = 0.008）相比也是如此。此外，与野生型（CC）基因型相比，rs1800896 CT 和 TT 基因型的渗出性 AMD 患者的 IL-10 血清浓度较低（ = 0.048）。总之，我们的研究表明，IL-10 血清水平可能与 rs1800896 的次要等位基因和渗出性 AMD 相关。IL-9 SNP 的单倍型也与早期和渗出性 AMD 发生的几率降低相关。
