Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI- 1230 Domzale, Slovenia.
Int J Biol Sci. 2010 Dec 2;6(7):769-72. doi: 10.7150/ijbs.6.769.
Mir717 (mmu-mir-717) was first reported in mouse and resides in the intron 3 of glypican 3 (Gpc3) gene. Our present study revealed that this microRNA (miRNA) gene is conserved among 26 mammalian species and harbors polymorphic sites within the mature seed region in mice. Our finding represents a rare four layer genomic overlap consisting of growth associated quantitative trait locus (QTL), body mass associated Gpc3 gene, highly conserved miRNA gene and mature miRNA seed single nucleotide polymorphism (SNP) identified in the lean mouse strain 129/Sv. Additionally, genes potentially targeted by Mir717 include 91 genes associated with obesity and related phenotypes in mammals. Our analysis provides a basis for further experiments to causally connect the identified SNP and Mir717 gene itself to obesity regulation. Furthermore, our bioinformatics analysis now enables functional annotation of Mir717 orthologs in other species, thus determining the effect of its target genes on fat-related traits.
Mir717（mmu-mir-717）最初在小鼠中被报道，位于聚糖蛋白 3（Gpc3）基因的内含子 3 中。我们的研究揭示了这种 microRNA（miRNA）基因在 26 种哺乳动物中是保守的，并且在小鼠的成熟种子区域存在多态性位点。我们的发现代表了一个罕见的四层基因组重叠，包括与生长相关的数量性状位点（QTL）、与体重相关的 Gpc3 基因、高度保守的 miRNA 基因和在瘦鼠品系 129/Sv 中鉴定的成熟 miRNA 种子单核苷酸多态性（SNP）。此外，Mir717 可能靶向的基因包括 91 个与哺乳动物肥胖和相关表型相关的基因。我们的分析为进一步的实验提供了基础，以将鉴定的 SNP 和 Mir717 基因本身与肥胖调节联系起来。此外，我们的生物信息学分析现在可以对其他物种的 Mir717 同源物进行功能注释，从而确定其靶基因对与脂肪相关的特征的影响。
