College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, People's Republic of China.
J Appl Genet. 2013 May;54(2):209-13. doi: 10.1007/s13353-013-0142-4. Epub 2013 Mar 2.
In the present study, a total of 860 chickens from a Gushi-Anka F2 resource population were used to evaluate the genetic effect of the gga-miR-1614-3p gene. A novel, silent, single nucleotide polymorphism (SNP, +5 C>T) was detected in the gga-miR-1614-3p gene seed region through AvaII polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PCR products sequencing methods. Associations between the SNP and chicken growth, meat quality and carcass traits were performed by association analysis. The results showed that the SNP was significantly associated with breast muscle shear force and leg muscle water loss rate, wing weight, liver weight and heart weight (p<0.05), and highly significantly associated with the weight of the abdominal fat (p<0.01). The secondary structure of gga-miR-1614 and the free energy were altered due to the variation predicted by the M-fold program.
本研究以固始鸡 F2 资源群体为试验材料，利用 860 只鸡对gga-miR-1614-3p 基因的遗传效应进行了评估。通过 AvaII 聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和 PCR 产物测序方法，在gga-miR-1614-3p 基因的种子区检测到一个新的、沉默的单核苷酸多态性（SNP，+5C>T）。通过关联分析，研究了 SNP 与鸡生长、肉质和胴体性状的关系。结果表明，该 SNP 与胸肌剪切力和腿肌失水率、翅膀重量、肝脏重量和心脏重量显著相关（p<0.05），与腹脂重量高度显著相关（p<0.01）。M-fold 程序预测的变异导致 gga-miR-1614 的二级结构和自由能发生改变。
