Pinto Vicente P T, Debray Henri, Dus Danuta, Teixeira Edson H, de Oliveira Taianá Maia, Carneiro Victor Alves, Teixeira Alrieta H, Filho Gerardo C, Nagano Celso S, Nascimento Kyria S, Sampaio Alexandre H, Cavada Benildo S
Curso de Medicina/Campus de Sobral, Universidade Federal do Ceará, Fortaleza 60020-181, Brazil.
Adv Pharmacol Sci. 2009;2009:862162. doi: 10.1155/2009/862162. Epub 2009 Dec 9.
The carbohydrate-binding activity of the algal lectins from the closely related red marine algal species Bryothamnion triquetrum (BTL) and Bryothamnion seaforthii (BSL) was used to differentiate human colon carcinoma cell variants with respect to their cell membrane glyco-receptors. These lectins interacted with the cells tested in a dose-dependent manner. Moreover, the fluorescence spectra of both lectins clearly differentiated the cells used as shown by FACS profiles. Furthermore, as observed by confocal microscopy, BTL and BSL bound to cell surface glycoproteins underwent intense internalization, which makes them possible tools in targeting strategies.
来自亲缘关系相近的红色海洋藻类三角叉节藻（BTL）和海氏叉节藻（BSL）的藻类凝集素的碳水化合物结合活性，被用于区分人结肠癌细胞变体的细胞膜糖受体。这些凝集素与受试细胞以剂量依赖方式相互作用。此外，两种凝集素的荧光光谱如流式细胞仪图谱所示，能清晰区分所用细胞。而且，通过共聚焦显微镜观察发现，与细胞表面糖蛋白结合的BTL和BSL会发生强烈的内化作用，这使它们成为靶向策略中的潜在工具。
