具有 IDA 电极的寻址电极阵列器件，用于高通量检测。

Addressable electrode array device with IDA electrodes for high-throughput detection.

机构信息

Graduate School of Environmental Studies, Tohoku University, Sendai, Japan.

出版信息

Lab Chip. 2011 Feb 7;11(3):385-8. doi: 10.1039/c0lc00437e. Epub 2010 Dec 13.

Abstract

An electrochemical device is proposed for high-throughput electrochemical detection that consists of 32 row and 32 column electrodes on a single glass substrate. The row and column electrodes are connected to interdigitated array (IDA) electrodes to form 1024 (32 × 32) addressable sensor points in the device. Electrochemical responses from each of the 1024 sensors were successfully acquired on the device within 1 min using redox cycling at individual IDA electrodes, which ensures application of the device to comprehensive, high-throughput electrochemical detection for enzyme-linked immunosorbent assay (ELISA), reporter gene assay for monitoring gene expressions, and DNA analysis.

摘要

本文提出了一种用于高通量电化学检测的电化学装置，该装置由单个玻璃衬底上的 32 行和 32 列电极组成。行和列电极与叉指式阵列（IDA）电极相连，在器件中形成 1024（32×32）个可寻址传感器点。通过在单个 IDA 电极上进行氧化还原循环，成功地在 1 分钟内从该器件上获得了 1024 个传感器中的每个传感器的电化学响应，这确保了该器件可应用于酶联免疫吸附测定（ELISA）的高通量全面电化学检测、用于监测基因表达的报告基因测定以及 DNA 分析。