Sousa Saulo M, Reis Aryane C, Gomes Shaiany S L, Bernardo Karina B, Salimena Fátima R G, Viccini Lyderson F
Instituto de Ciências Biológicas, Departamento de Biologia, Laboratório de Genética, Universidade Federal de Juiz de Fora, Campus Universitário, Bairro Martelos, Juiz de Fora, MG, Brasil.
An Acad Bras Cienc. 2010 Dec;82(4):868-79. doi: 10.1590/s0001-37652010000400009.
The genus Heteropterys is one of the major genera in Malpighiaceae. However, few cytological and palynological studies were reported. The present work described for the first time the chromosome number, heterochromatin pattern, meiotic behavior, pollen viability and palynological aspects of Heteropterys ubellata, a very spread species. One large Brazilian population was evaluated using conventional techniques for meiotic studies and acetolyse to access the pollen morphology. The species showed 2n = 20 chromosomes, normal meiotic development and viable pollens. Great blocks of heterochromatin were observed around the centromers. DAPI staining was positive for centroeric heterochroatin, hile CMA3 ark as observed just at terinal regions of one pair of hoologues chromosomes. This result and the presence of one chromosome pair attached to the nucleoli during the pachytene and diakinesis suggested the presence of only one pair of NORs. Palynological analysis revealed that pollen grains are apolar, 6 porate and with colpoids associated to all pores. The pollen content was positive for the starch test, and the exine was rugulate with little psilate regions.
异翅藤属是金虎尾科的主要属之一。然而,关于其细胞学和孢粉学的研究报道较少。本研究首次描述了分布广泛的伞花异翅藤的染色体数目、异染色质模式、减数分裂行为、花粉活力及孢粉学特征。利用传统技术对巴西的一个大种群进行减数分裂研究,并采用醋酸分解法观察花粉形态。该物种染色体数目为2n = 20,减数分裂发育正常,花粉有活力。在着丝粒周围观察到大量异染色质。DAPI染色显示着丝粒异染色质呈阳性,而CMA3染色仅在一对同源染色体的末端区域观察到。这一结果以及在粗线期和终变期有一对染色体与核仁相连,表明仅存在一对核仁组织区。孢粉学分析表明,花粉粒无极性,具6孔,所有孔均有拟孔。花粉内容物淀粉测试呈阳性,外壁具皱状,有少量光滑区域。