Faculté de Médecine, EA 3842, Homéostasie Cellulaire et Pathologies, 87025 Limoges, France.
Int J Oncol. 2011 Feb;38(2):391-9. doi: 10.3892/ijo.2010.862. Epub 2010 Dec 6.
The P75 neurotrophin receptor (p75NTR) is a cell surface receptor that can induce apoptosis in many cell types. This receptor plays a major role in the development of the central nervous system and is expressed in some adult brain cells. Its implication in cell apoptosis or survival is probably of major importance in cellular homeostasis and thus p75NTR could be implicated in tumor resistance to death. In this study, we investigated the intracellular expression of p75NTR in a human glioblastoma cell line. Detection of p75NTR receptor in Golgi apparatus by immunofluorescence microscopy, or after Golgi apparatus extraction, could be correlated with a decrease of cell apoptosis leading cells to become tumorous. This hypothesis is supported by a loss of ligand-induced apoptosis in this cell line. Our observations show that p75NTR can be sequestered in the Golgi complex and could then be, in part, responsible for the cell resistance to apoptosis and for brain tumor formation.
P75 神经生长因子受体（p75NTR）是一种细胞表面受体，可诱导许多细胞类型的凋亡。该受体在中枢神经系统的发育中起主要作用，并在一些成年脑细胞中表达。它在细胞凋亡或存活中的作用可能对细胞内稳态非常重要，因此 p75NTR 可能与肿瘤对死亡的抵抗有关。在这项研究中，我们研究了人神经胶质瘤细胞系中 p75NTR 的细胞内表达。通过免疫荧光显微镜检测高尔基体内的 p75NTR 受体，或在高尔基体制备后进行检测，与细胞凋亡减少相关，从而导致肿瘤形成。这一假说得到了该细胞系中配体诱导的凋亡缺失的支持。我们的观察表明，p75NTR 可以被隔离在高尔基复合体中，并且可能部分导致细胞对凋亡的抵抗和脑肿瘤的形成。
