Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan.
Eur J Nutr. 2011 Sep;50(6):427-36. doi: 10.1007/s00394-010-0149-0. Epub 2010 Dec 9.
Plasmalogen is a subclass of phospholipids widely distributed in animal tissues and ingested as food; however, the absorptive characteristics of different classes of plasmalogen have not been clarified.
Our object was to compare the lymphatic output of choline and ethanolamine plasmalogens after an administration of phospholipid preparations containing each class of plasmalogens, and to analyze molecular species of plasmalogen absorbed into the lymph.
A duodenal infusion of 1 ml of 10% emulsion of choline phospholipid (PC) containing 50.6% choline plasmalogen (PlsCho) or ethanolamine phospholipid (PE) containing 52.5% ethanolamine plasmalogen (PlsEtn) was administered in the lymph duct-cannulated rats. Molecular species of plasmalogen absorbed into the lymph were measured by LC-MS/MS.
Lymph outputs of PlsCho and PlsEtn increased and reached a peak value at 3 h after PC and PE injection, respectively. The peak value of PlsCho was much higher and remained at a high level until 8 h, whereas PlsEtn output fell to half of the peak value at 7 h. Total lymphatic output of PlsCho was 5-times higher than that of PlsEtn. Compositions of sn-1 in lymph plasmalogens roughly reflected those of the injected lipids, whereas sn-2 in both PlsCho and PlsEtn was rich in arachidonic acid (20:4) regardless of the composition of the administered fatty acid. Both plasmalogen and lysoplasmalogen after PE injection were not released into the portal vein.
Lymphatic absorption of PlsCho is much higher than that of PlsEtn in rats, and plasmalogens are re-esterified as 20:4-rich forms in the small intestine.
磷脂质是广泛分布于动物组织并作为食物摄入的磷脂的一个亚类；然而，不同类别的磷脂质的吸收特征尚未阐明。
我们的目的是比较给予含有各类型磷脂质的磷脂质制剂后，胆堿和乙醇胺磷脂质的淋巴输出，并分析吸收到淋巴中的磷脂质的分子种类。
在淋巴导管插管大鼠中，十二指肠内输注 1 毫升含有 50.6%胆堿磷脂质（PC）的 10%乳剂，其中含有 50.6%胆堿磷脂质（PlsCho）或含有 52.5%乙醇胺磷脂质（PE）的 10%乳剂，其中含有 52.5%乙醇胺磷脂质（PlsEtn）。通过 LC-MS/MS 测量吸收到淋巴中的磷脂质的分子种类。
PlsCho 和 PlsEtn 的淋巴输出增加，并在 PC 和 PE 注射后 3 小时达到峰值。PlsCho 的峰值高得多，并保持在高水平，直到 8 小时，而 PlsEtn 的输出在 7 小时下降到峰值的一半。PlsCho 的总淋巴输出是 PlsEtn 的 5 倍。淋巴中磷脂质的 sn-1 组成大致反映了注入脂质的组成，而无论给予的脂肪酸组成如何，PlsCho 和 PlsEtn 的 sn-2 都富含花生四烯酸（20:4）。PE 注射后的磷脂质和溶血磷脂质都不会释放到门静脉。
大鼠中 PlsCho 的淋巴吸收远高于 PlsEtn，并且在小肠中重新酯化形成富含 20:4 的形式。
