激光脉冲或渗透压休克法对玻璃化前人类囊胚的体外和体内活力的影响。
In vitro and in vivo viability of human blastocysts collapsed by laser pulse or osmotic shock prior to vitrification.
机构信息
Yamashita Ladies' Clinic, Kobe, Hyogo, 651-0086, Japan.
出版信息
J Assist Reprod Genet. 2011 Apr;28(4):355-61. doi: 10.1007/s10815-010-9522-4. Epub 2010 Dec 9.
Abstract
PURPOSE
This study was designed to investigate whether artificial shrinkage, induced by a laser pulse or hyperosmotic sucrose solutions, improves in vitro survival and/or implantation of vitrified-warmed human expanded blastocysts.
METHODS
Before Cryotop vitrification, the blastocoelic cavity was collapsed either by a laser pulse or sucrose solutions. Non-treated blastocysts were used as control. Post-warm blastocyst survival and implantation after transfer were examined. Implantation rate outcome was retrospectively analyzed by morphological grading and developmental kinetics of post-warm blastocysts.
RESULTS
Survival rates in the three groups were high. Implantation rates in the laser-pulse group (59.7%) were comparable with those in the sucrose group (49.3%), and were significantly higher than those in the control group (34.2%). The proportion of blastocysts showing fast development tended to be higher when the blastocysts underwent artificial shrinkage treatment before vitrification. There was no clear correlation between morphology of post-warm blastocysts and implantation rate.
CONCLUSION
Artificial shrinkage treatment before vitrification is associated with an increased probability of fast-developing embryos, resulting in higher implantation rates.
摘要
目的
本研究旨在探讨激光脉冲或高渗蔗糖溶液诱导的人工收缩是否能提高玻璃化冷冻-解冻的人扩张囊胚的体外存活率和/或植入率。
方法
在 Cryotop 玻璃化冷冻之前，通过激光脉冲或蔗糖溶液使囊胚腔塌陷。未处理的囊胚作为对照。检测解冻后囊胚的存活和移植后的植入情况。通过形态学分级和解冻后囊胚的发育动力学回顾性分析植入率的结果。
结果
三组的存活率均较高。激光脉冲组（59.7%）的植入率与蔗糖组（49.3%）相当，明显高于对照组（34.2%）。在玻璃化冷冻前进行人工收缩处理的囊胚中，快速发育的囊胚比例较高。解冻后囊胚的形态与植入率之间没有明显的相关性。
结论
玻璃化冷冻前的人工收缩处理与快速发育胚胎的可能性增加有关，从而导致更高的植入率。
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