College of Biological and Environmental Sciences, Zhejiang Wanli University, No.8, South Qianhu Road, Ningbo 315100, Zhejiang Province, China.
Arch Virol. 2011 Mar;156(3):523-8. doi: 10.1007/s00705-010-0852-4. Epub 2010 Dec 10.
This is the first sequence-based characterization of mud crab (Scylla serrata) reovirus (SsRV), which causes severe disease of cultured mud crabs in southern China. We sequenced and analyzed genome segments S1, S2, S3, and S7, which were 4,327, 2,721, 2,715, and 1,517 nucleotides long, respectively. Conserved motifs were found at the 5' (AUAAAU) and 3' (AACGAU) ends of each segment. RNA segments S1, S2, S3, and S7 each contained a single open reading frame (ORF) that encoded predicted proteins of 160, 100, 96, and 46 kDa, respectively. The ORFs of segments S1 and S2 showed distant homologies (< 25%) with cognate genes of other reoviruses, whereas the ORFs of segments S3 and S7 had no homologies with any other viral genes. Based on these observations, we propose that SsRV should be considered a member of a new genus in the family Reoviridae.
这是对泥蟹虹彩病毒（SsRV）进行的基于序列的首次特征描述，该病毒可引起中国南方养殖泥蟹的严重疾病。我们对基因组片段 S1、S2、S3 和 S7 进行了测序和分析，它们分别长 4327、2721、2715 和 1517 个核苷酸。在每个片段的 5'（AUAAAU）和 3'（AACGAU）端都发现了保守的基序。RNA 片段 S1、S2、S3 和 S7 分别包含一个单一的开放阅读框（ORF），编码预测的 160、100、96 和 46 kDa 蛋白。片段 S1 和 S2 的 ORF 与其他呼肠孤病毒的同源基因具有较远的同源性（<25%），而片段 S3 和 S7 的 ORF 与任何其他病毒基因均无同源性。基于这些观察结果，我们建议将 SsRV 视为呼肠孤病毒科的一个新属成员。
