Flowers Emily M, Bachvaroff Tsvetan R, Warg Janet V, Neill John D, Killian Mary L, Vinagre Anapaula S, Brown Shanai, Almeida Andréa Santos E, Schott Eric J
Institute of Marine and Environmental Technology, University of Maryland Center for Environmental ScienceBaltimore, MD USA; University of Maryland School of MedicineBaltimore, MD USA.
Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science Baltimore, MD USA.
Front Microbiol. 2016 Feb 10;7:126. doi: 10.3389/fmicb.2016.00126. eCollection 2016.
The blue crab, Callinectes sapidus Rathbun, 1896, which is a commercially important trophic link in coastal ecosystems of the western Atlantic, is infected in both North and South America by C. sapidus Reovirus 1 (CsRV1), a double stranded RNA virus. The 12 genome segments of a North American strain of CsRV1 were sequenced using Ion Torrent technology. Putative functions could be assigned for 3 of the 13 proteins encoded in the genome, based on their similarity to proteins encoded in other reovirus genomes. Comparison of the CsRV1 RNA-dependent RNA polymerase (RdRP) sequence to genomes of other crab-infecting reoviruses shows that it is similar to the mud crab reovirus found in Scylla serrata and WX-2012 in Eriocheir sinensis, Chinese mitten crab, and supports the idea that there is a distinct "Crabreo" genus, different from Seadornavirus and Cardoreovirus, the two closest genera in the Reoviridae. A region of 98% nucleotide sequence identity between CsRV1 and the only available sequence of the P virus of Macropipus depurator suggests that these two viruses may be closely related. An 860 nucleotide region of the CsRV1 RdRP gene was amplified and sequenced from 15 infected crabs collected from across the geographic range of C. sapidus. Pairwise analysis of predicted protein sequences shows that CsRV1 strains in Brazil can be distinguished from those in North America based on conserved residues in this gene. The sequencing, annotation, and preliminary population metrics of the genome of CsRV1 should facilitate additional studies in diverse disciplines, including structure-function relationships of reovirus proteins, investigations into the evolution of the Reoviridae, and biogeographic research on the connectivity of C. sapidus populations across the Northern and Southern hemispheres.
蓝蟹（学名：Callinectes sapidus Rathbun，1896年）是西大西洋沿海生态系统中具有重要商业价值的营养环节，在北美洲和南美洲均受到一种双链RNA病毒——蓝蟹呼肠孤病毒1型（CsRV1）的感染。利用离子激流技术对北美一株CsRV1的12个基因组片段进行了测序。根据基因组中编码的13种蛋白质与其他呼肠孤病毒基因组中编码蛋白质的相似性，可为其中3种蛋白质确定推定功能。将CsRV1的RNA依赖性RNA聚合酶（RdRP）序列与其他感染蟹类的呼肠孤病毒基因组进行比较，结果表明它与锯缘青蟹中发现的泥蟹呼肠孤病毒以及中华绒螯蟹（中国绒螯蟹）中的WX - 2012相似，支持存在一个与呼肠孤病毒科中最接近的两个属——东南亚病毒属和心脏呼肠孤病毒属不同的独特“蟹呼肠孤病毒属”的观点。CsRV1与清洁长腹蟹P病毒唯一可用序列之间98%的核苷酸序列同一性区域表明，这两种病毒可能密切相关。从在蓝蟹地理分布范围内采集的15只受感染蟹中扩增并测序了CsRV1 RdRP基因的一个860核苷酸区域。对预测蛋白质序列的成对分析表明，巴西的CsRV1菌株与北美洲的菌株可根据该基因中的保守残基加以区分。CsRV1基因组的测序、注释和初步群体指标应有助于开展不同学科的更多研究，包括呼肠孤病毒蛋白质的结构 - 功能关系研究、呼肠孤病毒科进化研究以及蓝蟹种群在南北半球连通性的生物地理学研究。
