Zhang Zhao, Yuan Yangyang, Fan Dongyang, Yang Jifang, Mao Zhijuan, Yan Yan, Chen Jigang
College of Biological and Environmental Sciences, Zhejiang Wanli University, No. 8, South Qianhu Road, Ningbo, 315100, Zhejiang, People's Republic of China.
Virus Genes. 2015 Aug;51(1):69-76. doi: 10.1007/s11262-015-1218-5. Epub 2015 Jun 24.
To promote viral entry, replication, release, and spread to neighboring cells, many cytolytic animal viruses encode proteins responsible for modification of host cell membrane permeability and for formation of ion channels in host cell membranes. Scylla serrata reovirus (SsRV) is a major pathogen that can severely damage mud crab (S. serrata) aquaculture. Protein p35, which is encoded by segment 10 of SsRV, contains two transmembrane domains. In this study, we found that SsRV p35 can induce membrane permeability changes when expressed in Escherichia coli. SsRV p35 expressed in bacterial cells existed as monomers under reducing conditions but formed homodimers and homotrimers under non-reducing conditions. These findings demonstrate that p35 may act as a viroporin; further studies are needed to elucidate the detailed structure-function relationships of this protein.
为促进病毒进入、复制、释放并扩散至邻近细胞，许多溶细胞性动物病毒编码负责改变宿主细胞膜通透性以及在宿主细胞膜上形成离子通道的蛋白质。锯缘青蟹呼肠孤病毒（SsRV）是一种可严重损害锯缘青蟹养殖业的主要病原体。由SsRV第10节段编码的p35蛋白含有两个跨膜结构域。在本研究中，我们发现SsRV p35在大肠杆菌中表达时可诱导膜通透性改变。在细菌细胞中表达的SsRV p35在还原条件下以单体形式存在，但在非还原条件下形成同源二聚体和同源三聚体。这些发现表明p35可能作为一种病毒离子通道蛋白；需要进一步研究以阐明该蛋白详细的结构-功能关系。
