Department of Pharmaceutical Sciences & Drug Research, Punjabi University, Patiala 147002, India.
J Nat Med. 2011 Apr;65(2):282-92. doi: 10.1007/s11418-010-0486-6. Epub 2010 Dec 10.
This study was designed to investigate the therapeutic potential of hydroalcoholic extracts of Acorus calamus (AC) in tibial and sural nerve transection (TST)-induced neuropathic pain in rats. The hot plate, paw heat allodynia, acetone drop, and pinprick tests were performed to assess the degree of heat hyperalgesia, heat and cold allodynia, and mechanical hyperalgesia, respectively, at different time intervals, i.e., day 0, 1, 3, 6, 9, 12, 15, 18, and 21. The tissue superoxide anion and total calcium were measured as markers of oxidative stress. Tissue myeloperoxidase activity was measured as a specific marker of inflammation. Histopathological evaluation was also performed in the nervous tissue to assess the axonal degeneration. Pregabalin served as positive control in this study. TST in rats significantly induced thermal hyperalgesia and allodynia, mechanical hyperalgesia, and increased the levels of superoxide anion, total calcium, and myeloperoxidase (MPO) activity. Moreover significant histological changes were also observed. Oral administration of AC hydroalcoholic extract (100 and 200 mg/kg for 14 days) attenuated TST-induced behavioral, biochemical, and histological changes. Acorus calamus has ameliorative potential in TST-induced painful neuropathy, and this effect may be attributed to its multiple actions including anti-inflammatory, antioxidant, and neuroprotective actions.
这项研究旨在探究菖蒲水醇提取物在大鼠胫神经和腓总神经切断（TST）诱导的神经性疼痛中的治疗潜力。使用热板、足底热痛觉过敏、丙酮滴和针刺痛测试分别在不同时间点（即第 0、1、3、6、9、12、15、18 和 21 天）评估热超敏反应、热和冷痛觉过敏以及机械性痛觉过敏的程度。组织中超氧阴离子和总钙被用作氧化应激的标志物进行测量。组织髓过氧化物酶活性被用作炎症的特异性标志物进行测量。还对神经组织进行了组织病理学评估以评估轴突变性。普瑞巴林在这项研究中作为阳性对照。大鼠 TST 显著诱导热超敏反应和痛觉过敏、机械性痛觉过敏，并增加超氧阴离子、总钙和髓过氧化物酶（MPO）活性的水平。此外，还观察到明显的组织学变化。连续 14 天口服给予菖蒲水醇提取物（100 和 200mg/kg）可减轻 TST 诱导的行为、生化和组织学变化。菖蒲具有改善 TST 诱导的痛性神经病变的潜力，这种作用可能归因于其多种作用，包括抗炎、抗氧化和神经保护作用。
