Cell and Molecular Biology Department, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia.
Mol Biotechnol. 2011 Jun;48(2):156-64. doi: 10.1007/s12033-010-9356-4.
In this study, we report the molecular characterization of clone Eg707 isolated from cell suspension culture of the oil palm. The deduced polypeptide of clone Eg707 is highly similar to an unknown protein from Arabidopsis thaliana. The presence of an Ald-Xan-dh-C2 superfamily domain in the deduced protein sequence suggested that Eg707 protein might be involved in abscisic acid biosynthesis. Eg707 might be present as a single copy gene in the oil palm genome. This gene is highly expressed in tissue cultured materials compared to vegetative and reproductive tissues, suggesting a role of this gene during oil palm somatic embryogenesis or at the early stages of embryo development. Expression analysis of Eg707 by RNA in situ hybridization showed that Eg707 transcripts were present throughout somatic embryo development starting from proembryo formation at the embryogenic callus stages till the maturing embryo stages. Since proembryo formation within the embryogenic callus is one of the first key factors in oil palm somatic embryo development, it is suggested that Eg707 could be used as a reliable molecular marker for detecting early stage of oil palm somatic embryogenesis.
在这项研究中，我们报告了从油棕悬浮细胞培养物中分离的克隆 Eg707 的分子特征。克隆 Eg707 的推断多肽与拟南芥中的一种未知蛋白高度相似。推断蛋白序列中存在 Ald-Xan-dh-C2 超家族结构域表明，Eg707 蛋白可能参与脱落酸的生物合成。Eg707 可能作为油棕基因组中的单拷贝基因存在。与营养组织和生殖组织相比，该基因在组织培养材料中高度表达，表明该基因在油棕体细胞胚胎发生或胚胎发育的早期阶段发挥作用。通过 RNA 原位杂交的表达分析表明，Eg707 转录本存在于体细胞胚胎发育的整个过程中，从胚性愈伤组织阶段的原胚形成开始，直到成熟胚胎阶段。由于胚性愈伤组织内的原胚形成是油棕体细胞胚胎发生的第一个关键因素之一，因此，Eg707 可以用作检测油棕体细胞胚胎发生早期阶段的可靠分子标记。
