Chavrier P, Parton R G, Hauri H P, Simons K, Zerial M
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Cell. 1990 Jul 27;62(2):317-29. doi: 10.1016/0092-8674(90)90369-p.
A set of 11 clones encoding putative GTP binding proteins highly homologous to the yeast YPT1/SEC4 gene products have been isolated from an MDCK cell cDNA library. We localized three of the corresponding proteins in mammalian cells by using affinity-purified antibodies in immunofluorescence and immunoelectron microscopy studies. One, the MDCK homolog of rab2, is associated with a structure having the characteristics of an intermediate compartment between the endoplasmic reticulum and the Golgi apparatus. The second, rab5, is located at the cytoplasmic surface of the plasma membrane and on early endosomes, while the third, rab7, is found on late endosomes. These findings provide evidence that members of the YPT1/SEC4 subfamily of GTP binding proteins are localized to specific exocytic and endocytic subcompartments in mammalian cells.
从MDCK细胞cDNA文库中分离出一组11个克隆，它们编码的假定GTP结合蛋白与酵母YPT1/SEC4基因产物高度同源。我们通过免疫荧光和免疫电子显微镜研究，使用亲和纯化抗体在哺乳动物细胞中定位了三种相应的蛋白质。一种是rab2的MDCK同源物，与具有内质网和高尔基体之间中间区室特征的结构相关。第二种是rab5，位于质膜的细胞质表面和早期内体上，而第三种是rab7，存在于晚期内体上。这些发现提供了证据，表明GTP结合蛋白的YPT1/SEC4亚家族成员定位于哺乳动物细胞中的特定胞吐和胞吞亚区室。
