Instituto de Salud Carlos III, Centro Nacional de Microbiologia, Servicio de Bacteriologia, Laboratorio de Campylobacter, Majadahonda, Madrid, Spain.
Avian Pathol. 2010 Dec;39(6):483-7. doi: 10.1080/03079457.2010.518138.
The aim of the present study was the molecular identification of a common source of infection of Campylobacter coli in two grandparent breeder farms. Campylobacter jejuni and C. coli were isolated from well water and cloacal swabs from grandparent chickens. Colonies were genotyped using restriction fragment length polymorphism-flaA gene, pulsed field gel electrophoresis and multi-locus sequence typing. The same genotype of C. coli was found in both farms and in the well from which drinking water was supplied to the farms. The well water was epidemiologically linked as the source of C. coli infection. The molecular identification for epidemiological source-tracking of C. coli in breeder farms could aid in combating the colonization of this pathogen and therefore to reduce their incidence in human campylobacteriosis.
本研究的目的是鉴定两个祖代种鸡场中结肠弯曲杆菌感染的共同来源。从祖代鸡的井水和泄殖腔拭子中分离出空肠弯曲杆菌和结肠弯曲杆菌。使用限制性片段长度多态性-flaA 基因、脉冲场凝胶电泳和多位点序列分型对分离株进行基因分型。在两个农场以及供应农场饮用水的井水中均发现了相同基因型的结肠弯曲杆菌。井水被确定为结肠弯曲杆菌感染的来源，具有流行病学联系。对种鸡场中结肠弯曲杆菌的流行病学溯源的分子鉴定有助于对抗这种病原体的定植，从而降低人类弯曲杆菌病的发病率。
