Chuma T, Makino K, Okamoto K, Yugi H
Department of Veterinary Public Health, Faculty of Agriculture, Kagoshima University, Japan.
J Vet Med Sci. 1997 Nov;59(11):1011-5. doi: 10.1292/jvms.59.1011.
The incidence of Campylobacter jejuni and Campylobacter coli in broiler farms was 33.9% (19/56). C. jejuni-positive flocks accounted for 20.0% (17/85) and C. coli-positive ones was 4.7% (4/85). There were 14 patterns (fla type) of restriction fragment length polymorphism (RFLP) of flagellin A gene among these 22 strains of C. jejuni and C. coli including the standard strain C. jejuni ATCC 33560. Different fla types of Campylobacter were isolated from broilers in different growing cycles on the same farms. Four strains of C. jejuni were isolated from four breeder farms and four fla types of C. jejuni were detected from their progenies reared on growing farms. Three fla types of C. jejuni detected from the progenies were different from those of each breeder. Also, the other three fla types of C. jejuni were detected from different progenies of each growing farm during the next growing cycle. These findings indicate that the RFLP analysis may contribute to epidemiological studies of C. jejuni and C. coli contamination of broilers and suggest the risk of contamination with different types of Campylobacter in every growing cycle of broilers on the farm even on the same farm. They also supported that there was little likeliness of the vertical transmission of C. jejuni and C. coli from breeders to broilers.
空肠弯曲菌和结肠弯曲菌在肉鸡养殖场的发生率为33.9%（19/56）。空肠弯曲菌阳性鸡群占20.0%（17/85），结肠弯曲菌阳性鸡群占4.7%（4/85）。在这22株空肠弯曲菌和结肠弯曲菌（包括空肠弯曲菌标准菌株ATCC 33560）中，鞭毛蛋白A基因的限制性片段长度多态性（RFLP）有14种模式（fla型）。同一养殖场不同生长周期的肉鸡中分离出不同fla型的弯曲菌。从四个种鸡场分离出四株空肠弯曲菌，并在生长场饲养的子代中检测到四种fla型的空肠弯曲菌。从子代中检测到的三种空肠弯曲菌fla型与每个种鸡场的不同。此外，在下一个生长周期中，从每个生长场的不同子代中又检测到另外三种空肠弯曲菌fla型。这些发现表明，RFLP分析可能有助于对肉鸡空肠弯曲菌和结肠弯曲菌污染进行流行病学研究，并提示即使在同一养殖场，肉鸡的每个生长周期都存在感染不同类型弯曲菌的风险。研究结果还支持空肠弯曲菌和结肠弯曲菌从种鸡垂直传播给肉鸡的可能性很小。
