通过二维和脉冲场凝胶电泳分析酿酒酵母中的DNA复制。
Analysis of DNA replication in Saccharomyces cerevisiae by two-dimensional and pulsed-field gel electrophoresis.
作者信息
Ide Satoru, Kobayashi Takehiko
机构信息
National Institute of Genetics, Shizuoka, Japan.
出版信息
Curr Protoc Cell Biol. 2010 Dec;Chapter 22:Unit 22.14. doi: 10.1002/0471143030.cb2214s49.
PMID:21154549
Abstract
Two methods to analyze DNA replication in budding yeast are described here. One is two-dimensional (2D) gel electrophoresis, which has been used to analyze replication intermediates in yeast. The other method is pulsed-field gel electrophoresis (PFGE) that monitors the replication status of a chromosome. These are convenient and easy, and they do not require specialized equipment.
摘要
本文介绍了两种分析芽殖酵母中DNA复制的方法。一种是二维（2D）凝胶电泳，它已被用于分析酵母中的复制中间体。另一种方法是脉冲场凝胶电泳（PFGE），它可监测染色体的复制状态。这些方法方便易行，且不需要专门的设备。
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