Henan Key Laboratory for Esophageal Cancer Research, Department of Gastroenterology, The First Affiliated Hospital, College of Basic Medicine, Zhengzhou University, Zhengzhou 450052, Henan Province, China.
World J Gastroenterol. 2010 Dec 14;16(46):5901-6. doi: 10.3748/wjg.v16.i46.5901.
To investigate the relationship between human papillomavirus (HPV) infection and concurrent esophagus and gastric cardia cancer from the same patient (CC) and examine the significance of P16(INK4A) protein expression.
Polymerase chain reaction was used to detect the presence of HPV type16 (HPV16). The expression of P16(INK4A) protein was detected using immunohistochemistry.
Among the CC specimens, HPV16-DNA was found in eight cases of esophageal squamous cell carcinoma (ESCC) and five cases of gastric cardia adenocarcinoma (GCA), respectively (47% vs 29%), and two of both ESCC and GCA. P16(INK4A) was highly expressed in both ESCC and GCA. In the HPV-associated positive CC, higher P16(INK4A) expression was observed in the GCA than in the ESCC (75% vs 25%, P < 0.05).
HPV16 as a correlated risk factor may play an important role in the development of ESCC and GCA. P16(INK4A) may be a screening index in the HPV-associated carcinoma of gastric cardia.
探讨同一患者食管和贲门癌（CC）中人类乳头瘤病毒（HPV）感染的关系，并研究 P16（INK4A）蛋白表达的意义。
采用聚合酶链反应检测 HPV 型 16（HPV16）的存在。采用免疫组织化学法检测 P16（INK4A）蛋白的表达。
在 CC 标本中，食管鳞状细胞癌（ESCC）和贲门腺癌（GCA）中分别发现了 8 例和 5 例 HPV16-DNA（47%比 29%），并且在 ESCC 和 GCA 中各有 2 例。P16（INK4A）在 ESCC 和 GCA 中均高度表达。在 HPV 相关阳性 CC 中，GCA 中 P16（INK4A）的表达高于 ESCC（75%比 25%，P<0.05）。
HPV16 作为相关危险因素，可能在 ESCC 和 GCA 的发生发展中起重要作用。P16（INK4A）可能是 HPV 相关贲门癌的筛查指标。
