Infections and Immunoepidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, 6120 Executive Boulevard, Room 7070, Rockville, MD 20852-7248, USA.
Cancer Epidemiol Biomarkers Prev. 2010 Apr;19(4):1137-9. doi: 10.1158/1055-9965.EPI-10-0089. Epub 2010 Mar 23.
Thousands of people in central Asia die every year from gastric cardia adenocarcinoma (GCA). GCA arises in the transformation zone between the esophagus and the stomach, similar to cervical and oropharyngeal carcinoma, which arise in areas with transformation zone characteristics. The analogous biology of the gastric cardia to the cervix and oropharynx, where human papillomavirus (HPV) is known to cause cancer, raises the possibility that GCA could be a HPV-associated cancer. Given the availability of an effective HPV vaccine and its potential to prevent HPV-associated cancer, we decided to evaluate the prevalence of HPV DNA in GCA.
We collected tumor tissue from 144 histopathologically confirmed GCA patients at Yaocun Commune Hospital (Linxian, China), with rigorous attention to prevent DNA contamination. We tested for the presence of HPV DNA in fresh-frozen tumor specimens using PCR with sensitive L1-, E6-, and E7-based primers.
DNA was adequate, as indicated by beta-globin positivity, in 108 cases. Of these, all (100%; 95% confidence interval, 97-100%) were negative for HPV DNA.
These results suggest that HPV does not contribute to gastric cardia carcinogenesis in north central China.
Because GCA does not seem to be a HPV-associated cancer, prophylactic HPV vaccination is unlikely to affect rates of GCA in China.
中亚地区每年有数千人死于胃贲门腺癌（GCA）。GCA 发生在食管和胃的转化区，类似于发生在具有转化区特征的部位的宫颈癌和口咽癌。胃贲门与宫颈和口咽的生物学相似，已知人乳头瘤病毒（HPV）可导致癌症，这使得 GCA 可能是一种 HPV 相关癌症。鉴于 HPV 疫苗的有效性及其预防 HPV 相关癌症的潜力，我们决定评估 GCA 中 HPV DNA 的流行率。
我们从中国临县耀村公社医院（Linxian, China）的 144 名经组织病理学证实的 GCA 患者中收集肿瘤组织，严格注意防止 DNA 污染。我们使用基于 L1、E6 和 E7 的敏感 PCR 检测新鲜冷冻肿瘤标本中 HPV DNA 的存在。
108 例标本中，β-球蛋白阳性表明 DNA 充足。其中，所有标本（100%；95%置信区间，97-100%）均为 HPV DNA 阴性。
这些结果表明 HPV 不会导致中国中北部胃贲门癌的发生。
由于 GCA 似乎不是 HPV 相关癌症，预防性 HPV 疫苗接种不太可能影响中国 GCA 的发病率。
