Northwest Lipid Metabolism and Diabetes Research Laboratories, Department of Medicine, University of Washington, Seattle, WA 98109-4517, USA.
Clin Chim Acta. 2011 Mar 18;412(7-8):556-61. doi: 10.1016/j.cca.2010.12.003. Epub 2010 Dec 11.
Small, dense LDL (sdLDL) are highly atherogenic, and evidence indicates that sdLDL are useful predictors of cardiovascular disease. We evaluated a homogeneous method for the quantitative determination of sdLDL cholesterol (sdLDL-C) and compared it to the LDL-cholesterol profile obtained by density gradient ultracentrifugation (DGUC).
sdLDL-C was measured in plasma and in lipoprotein fractions obtained by DGUC using the sdLDL-EX "Seiken" kit.
sdLDL-C was only present in dense or intermediate LDL fractions obtained by DGUC. Plasma sdLDL-C levels were inversely corelated to the LDL relative floatation rate. The proportion of LDL-C that is sdLDL-C increases as a function of LDL density from a median of 19% for very buoyant LDL to 91% for very dense LDL particles. Plasma sdLDL-C level was significantly correlated with plasma triglyceride (TG) (n=840, r(s)=0.710, p<0.001). Among subjects with plasma TG>150 mg/dl, 75% had sdLDL-C>30 mg/dl, whereas among those with TG ≤ 150 mg/dl, only 17% had sdLDL-C>30 mg/dl.
This precise and fully automated method for measuring plasma levels of sdLDL-C will allow the analysis of large number of samples in routine laboratories which will facilitate the evaluation of the clinical usefulness of sdLDL as a risk factor for CHD.
小而密的 LDL（sdLDL）具有高度的致动脉粥样硬化性，并且有证据表明 sdLDL 是心血管疾病的有用预测指标。我们评估了一种定量测定 sdLDL 胆固醇（sdLDL-C）的均一方法，并将其与密度梯度超速离心（DGUC）获得的 LDL 胆固醇谱进行了比较。
使用 sdLDL-EX“Seiken”试剂盒，通过 DGUC 测定血浆和脂蛋白级分中的 sdLDL-C。
sdLDL-C 仅存在于 DGUC 获得的致密或中间 LDL 级分中。血浆 sdLDL-C 水平与 LDL 相对漂浮率呈负相关。sdLDL-C 占 LDL-C 的比例随 LDL 密度的增加而增加，从中密度 LDL 的中位数 19%增加到非常致密 LDL 颗粒的 91%。血浆 sdLDL-C 水平与血浆甘油三酯（TG）显著相关（n=840，r(s)=0.710，p<0.001）。在 TG>150 mg/dl 的受试者中，75%的 sdLDL-C>30 mg/dl，而在 TG≤150 mg/dl 的受试者中，只有 17%的 sdLDL-C>30 mg/dl。
这种精确且全自动的血浆 sdLDL-C 水平测定方法将允许在常规实验室中分析大量样本，这将有助于评估 sdLDL 作为 CHD 风险因素的临床实用性。
