Department of Plant Pathology, The Pennsylvania State University, University Park 16802; and United States Department of Agriculture (USDA), Agriculture Research Service (ARS), Systematic Botany and Mycology Laboratory, Rm. 304, Bldg. 011A, BARC- West, Beltsville, Maryland 20705-2350.
Mycologia. 2002 Jul-Aug;94(4):630-40. doi: 10.1080/15572536.2003.11833191.
The objectives of this study were to determine the phylogenetic relationships of species of Leptosphaeria and Phaeosphaeria and evaluate the phylogenetic significance of morphological characters of the teleomorph, anamorph, and host. Sequences of the entire ITS region, including the 5.8S rDNA, of 59 isolates representing 54 species were analyzed and the phylogeny inferred using parsimony and distance analyses. Isolates grouped into three well-supported clades. The results of this study support the separation of Phaeosphaeria from Leptosphaeria sensu stricto. Leptosphaeria bicolor and the morphologically similar Leptosphaeria taiwanensis formed a separate, well-supported clade. We conclude that peridial wall morphology, anamorph characteristics, and to a lesser extent host, are phylogenetically significant at the generic level. Ascospore and conidial morphology are taxonomically useful at the species level.
本研究的目的是确定菜豆壳球腔菌属和拟球腔菌属物种的系统发育关系，并评估其有性态、无性态和宿主形态特征的系统发育意义。对代表 54 个种的 59 个分离株的整个 ITS 区（包括 5.8S rDNA）序列进行了分析，并使用简约法和距离法推断了系统发育关系。分离株分为三个支持度较高的分支。本研究结果支持将拟球腔菌属从狭义的菜豆壳球腔菌属中分离出来。异色球腔菌和形态上相似的台湾球腔菌形成了一个独立的、支持度较高的分支。我们得出结论，壁囊壳形态、无性态特征，在一定程度上还有宿主，在属的水平上具有系统发育意义。子囊孢子和分生孢子形态在种的水平上具有分类学意义。
