Liu Lijuan, Yang Chunlin, Liang Fang, Li Chengsong, Zeng Qian, Han Shan, Li Shujiang, Liu Yinggao
College of Forestry, Sichuan Agricultural University, Chengdu, China.
National Forestry and Grassland Administration, Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River and Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, College of Forestry, Sichuan Agricultural University, Chengdu, China.
Front Microbiol. 2024 Sep 18;15:1456993. doi: 10.3389/fmicb.2024.1456993. eCollection 2024.
Bamboo resources have garnered significant global attention due to their excellent capacity for regeneration and high yield. Rhombic-spot disease, a substantial threat to fishscale bamboo (), is primarily caused by . This study first reported the genome assemblies and characteristics of two isolates using PacBio and Illumina sequencing platforms. The genomes of strain SICAUCC 16-0001 and strain SICAUCC 23-0140, with sizes of 48.0 Mb and 48.4 Mb, respectively, revealed 10,289 and 10,313 protein-coding genes. Additionally, they contained 34.99 and 34.46% repetitive sequences within AT-rich regions, with notable repeat-induced point mutation activity. Comparative genome analysis identified 1,049 contracted and 45 expanded gene families in the genome of , including several related to pathogenicity. Several gene families involved in mycotoxin metabolism, secondary metabolism, sterol biosynthesis and transport, and cell wall degradation were contracted. Compared to most analyzed necrotrophic, hemibiotrophic, and phaeosphaeriacous pathogens, the genomes of two isolates exhibited fewer secondary metabolite enzymes, carbohydrate-active enzymes, plant cell wall degrading enzymes, secreted proteins, and effectors. Comparative genomics analysis suggested that shares more similar characteristics with hemibiotrophic pathogens. Based on single carbon source tests, strains demonstrated a higher potential for xylan decomposition than pectin and cellulose. The proportion of cell wall-degrading enzyme effectors occupied a high proportion of the total effectors of the genomes. These findings provide valuable insights into uncovering the pathogenesis of toward the efficient management of rhombic-spot disease of fishscale bamboo.
竹资源因其出色的再生能力和高产特性而受到全球广泛关注。菱形斑病是对鱼肚腩竹（）的重大威胁，主要由 引起。本研究首次报道了使用PacBio和Illumina测序平台对两个 分离株的基因组组装及特征。菌株SICAUCC 16 - 0001和菌株SICAUCC 23 - 0140的基因组大小分别为48.0 Mb和48.4 Mb，分别揭示了10,289个和10,313个蛋白质编码基因。此外，它们在富含AT的区域含有34.99%和34.46%的重复序列，具有显著的重复诱导点突变活性。比较基因组分析在 的基因组中鉴定出1,049个收缩和45个扩张的基因家族，包括几个与致病性相关的家族。几个参与霉菌毒素代谢、次生代谢、甾醇生物合成和转运以及细胞壁降解的基因家族收缩。与大多数分析的坏死营养型、半活体营养型和球腔菌病原体相比，两个 分离株的基因组表现出较少的次生代谢物酶、碳水化合物活性酶、植物细胞壁降解酶、分泌蛋白和效应子。比较基因组学分析表明 与半活体营养型病原体具有更多相似特征。基于单碳源测试， 菌株对木聚糖分解的潜力高于果胶和纤维素。细胞壁降解酶效应子在 基因组的总效应子中占比很高。这些发现为揭示 对鱼肚腩竹菱形斑病的致病机制以实现有效管理提供了有价值的见解。
