Department of Plant Pathology, The Royal Horticultural Society, Wisley, Woking, Surrey GU23 6QB, United Kingdom.
Mycologia. 2002 Nov-Dec;94(6):980-97.
A leaf and twig blight disease of Buxus spp. was found to be associated with a new species of Cylindrocladium. The novel species status was confirmed using morphological characters, sequencing of the ribosomal 5.8S RNA gene and the flanking internal transcribed spacers (ITS), the β-tubulin gene, and the high mobility group (HMG) of the MAT2 mating type gene. Cylindrocladium buxicola is proposed as a new name. Fifteen isolates from the UK and one isolate from New Zealand were paired in all combinations but no fertile perithecia were obtained suggesting that C. buxicola is heterothallic and all isolates belonged to one mating type. AFLP analysis showed that the isolates collected in the UK and New Zealand are genetically homogenous. Phylogenetic analyses indicated that this species falls within a new lineage.
发现黄杨属植物的叶和小枝枯萎病与一种新的旋孢腔菌有关。使用形态特征、核糖体 5.8S RNA 基因和侧翼内部转录间隔区（ITS）、β-微管蛋白基因以及 MAT2 交配型基因的高迁移率组（HMG）对新种进行了确认。提出了旋孢腔菌黄杨新名称。来自英国的 15 个分离株和来自新西兰的 1 个分离株进行了所有组合的配对，但未获得可育的子囊壳，表明旋孢腔菌黄杨是异宗配合的，所有分离株都属于一种交配型。AFLP 分析表明，在英国和新西兰收集的分离株在遗传上是同质的。系统发育分析表明，该种属于一个新谱系。
