Henricot B, Gorton C, Denton G, Denton J
Plant Pathology Department, The Royal Horticultural Society, Wisley, Woking, Surrey, GU23 6QB, UK.
Plant Dis. 2008 Sep;92(9):1273-1279. doi: 10.1094/PDIS-92-9-1273.
Cylindrocladium buxicola is a fungal pathogen that causes a severe leaf and twig blight on Buxus spp. Laboratory experiments were carried out to study the in vitro effect of 13 fungicides on mycelial growth and conidia germination of the fungus. Based on the values of the effective concentration at which mycelial growth was inhibited by 50%, the fungicide Stroby (kresoxim-methyl) and the combined fungicides Opponent (epoxiconazole + kresoxim-methyl + pyraclostrobin), Opera (epoxiconazole + pyraclostrobin), and Signum (boscalid + pyraclostrobin) were the most effective at inhibiting mycelial growth and conidia germination. Pathogenicity assays showed that the host range of the fungus was not limited to the genus Buxus because Sarcococca sp. also was susceptible. None of the 10 boxwood species and cultivars tested were immune to the disease, although Buxus balearica as well as Sarcococca sp. showed significantly lower levels of infection as measured by the expression of leaf symptoms and the number of conidia produced on host tissue, the latter probably contributing to the apparent resistance of these species in the field. Microscopic observation showed that disease development was very rapid and aggressive on B. sempervirens 'Suffruticosa' where it was able to survive at least 5 years on decomposing fallen leaves.
柱盘孢属病菌是一种真菌病原体，可导致黄杨属植物出现严重的叶枯和枝枯病。开展了实验室实验，以研究13种杀菌剂对该真菌菌丝体生长和分生孢子萌发的体外作用。根据抑制菌丝体生长50%时的有效浓度值，杀菌剂翠贝（醚菌酯）以及复配杀菌剂敌力脱（戊唑醇+醚菌酯+吡唑醚菌酯）、稳歼菌（戊唑醇+吡唑醚菌酯）和健达（咯菌腈+吡唑醚菌酯）在抑制菌丝体生长和分生孢子萌发方面最为有效。致病性测定表明，该真菌的寄主范围不限于黄杨属，因为清香桂属植物也易感病。尽管从叶片症状表现和寄主组织上产生的分生孢子数量来看，西班牙黄杨以及清香桂属植物的感染水平显著较低，后者可能是这些物种在田间表现出明显抗性的原因，但所测试的10种黄杨属物种和品种中没有一个对该病免疫。显微镜观察表明，在‘金心黄杨’上病害发展非常迅速且具有侵袭性，在腐烂的落叶上它至少能存活5年。
