Violi F, Pratico D, Ghiselli A, Alessandri C, Iuliano L, Cordova C, Balsano F
Institute of Clinica Medica I, University of Rome, Policlinico Umberto I, Italy.
Atherosclerosis. 1990 Jun;82(3):247-52. doi: 10.1016/0021-9150(90)90046-l.
Platelet aggregation induced by threshold concentrations of agonists such as collagen, PAF or epinephrine was inhibited in vitro by 100 microM aspirin but was restored by stimulating platelets with high concentrations of collagen, PAF or by a combination of epinephrine and PAF. Incubating aspirin-treated platelets with 50-100 microM vitamin E or vitamin E acetate inhibited platelet aggregation by high concentrations of collagen and PAF and by the combination of epinephrine and PAF; platelet thromboxane A2 formation was less than 10% in samples incubated with 100 microM aspirin. Apyrase, added to aspirin-treated platelet, did not influence platelet aggregation induced by epinephrine and PAF. The present study suggests that concentrations of vitamin E as low as 50-100 microM inhibit cyclooxygenase-independent platelet aggregation when combined with an inhibitor of the arachidonate pathway.
在体外，100微摩尔的阿司匹林可抑制由阈值浓度的激动剂（如胶原蛋白、血小板活化因子或肾上腺素）诱导的血小板聚集，但通过用高浓度的胶原蛋白、血小板活化因子刺激血小板，或肾上腺素与血小板活化因子联合刺激，血小板聚集可恢复。用50 - 100微摩尔的维生素E或维生素E醋酸酯孵育经阿司匹林处理的血小板，可抑制高浓度的胶原蛋白和血小板活化因子以及肾上腺素与血小板活化因子联合诱导的血小板聚集；在与100微摩尔阿司匹林孵育的样本中，血小板血栓素A2的形成少于10%。向经阿司匹林处理的血小板中添加腺苷三磷酸双磷酸酶，并不影响由肾上腺素和血小板活化因子诱导的血小板聚集。本研究表明，当与花生四烯酸途径的抑制剂联合使用时，低至50 - 100微摩尔的维生素E浓度可抑制不依赖环氧化酶的血小板聚集。
