Yan Jin-Hua, Li Zhan-Quan, Ji Lin-Hua, Chai Ke-Xia, Ge Ri-Li
Department of Hematology, Qinghai University Affiliated Hospital, Qinghai 810001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2009 Nov;25(4):457-60.
The clinical manifestation of chronic mountain sickness (CMS) is polycythemia, pulmonary hypertension and mionectic blood. However, the pathogenesis of it is not identified now. So it is necessary to investigate the effects of the angiogenic growth factors on the pathophysiologic development of CMS.
The serum levels of basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) in 13 healthy Tibetan natives (Native), 17 healthy people in Xining (control group) and 35 CMS patients were determined by quantitative sandwich enzyme immunoassay. Meanwhile, the levels of Hb, Hct and SaO2 were determined.
The serum levels of bFGF (107.26 +/- 7.86) ng/L, PDGF (630.18 +/- 9.89) ng/L and VEGF (543.74 +/- 6.76) ng/L in CMS were significantly higher than those in Natives (37.01 +/- 9.16; 292.16 +/- 6.88; 125.51 +/- 7.26) ng/L, and in control group (40.58 +/- 5.34; 287.68 +/- 8.33; 76.26 +/- 4.60) ng/L, respectively (P < 0.01). There was no difference between the natives and the control group in bFGF and PDGF (P > 0.05), while there was predominant difference between the Natives and the control group in VEGF (P < 0.01). There was a predominant positive correlation between the serum levels of bFGF, PDGF or VEGF and hemoglobin concentrations in CMS respectively (P < 0.01). And there were positive relations between angiogenic growth factors each other.
The serum levels of bFGF, PDGF and VEGF in patients with CMS significantly increase, these angiogenic growth factors may play important role on the pathophysiologic development of CMS; the VEGF level likely contributes to the adaptation to plateau hypoxia in healthy Tibetan natives; the elevated bFGF, PDGF and VEGF levels are likely associated with excessive erythropoiesis in CMS.
慢性高原病（CMS）的临床表现为红细胞增多、肺动脉高压和血液黏稠。然而，其发病机制目前尚未明确。因此，有必要研究血管生成生长因子对CMS病理生理发展的影响。
采用定量夹心酶免疫分析法测定13名健康藏族本地人（本地人组）、17名西宁健康人（对照组）和35例CMS患者血清中碱性成纤维细胞生长因子（bFGF）、血小板衍生生长因子（PDGF）和血管内皮生长因子（VEGF）的水平。同时，测定血红蛋白（Hb）、血细胞比容（Hct）和动脉血氧饱和度（SaO₂）水平。
CMS患者血清中bFGF（107.26±7.86）ng/L、PDGF（630.18±9.89）ng/L和VEGF（543.74±6.76）ng/L水平显著高于本地人组（37.01±9.16；292.16±6.88；125.51±7.26）ng/L和对照组（40.58±5.34；287.68±8.33；76.26±4.60）ng/L（P<0.01）。本地人组与对照组bFGF和PDGF水平无差异（P>0.05），而VEGF水平在本地人组与对照组之间存在显著差异（P<0.01）。CMS患者血清中bFGF、PDGF或VEGF水平与血红蛋白浓度分别呈显著正相关（P<0.01）。并且血管生成生长因子之间存在正相关关系。
CMS患者血清中bFGF、PDGF和VEGF水平显著升高，这些血管生成生长因子可能在CMS的病理生理发展中起重要作用；VEGF水平可能有助于健康藏族本地人适应高原低氧环境；bFGF、PDGF和VEGF水平升高可能与CMS中红细胞生成过多有关。
