Al-Kappany Y M, Rajendran C, Abu-Elwafa S A, Hilali M, Su C, Dubey J P
U.S. Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350, USA.
J Parasitol. 2010 Dec;96(6):1112-4. doi: 10.1645/GE-2608.1. Epub 2010 Aug 20.
Cats are important in the epidemiology of Toxoplasma gondii because they are the only hosts that excrete environmentally resistant oocysts in feces. In the present study, 115 viable T. gondii isolates from tissues of cats from Egypt were genotyped using 10 PCR-restriction fragment length polymorphism markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) and DNA from tachyzoites. Seven genotypes were recognized including the clonal Type II, Type III (2 genotypes), and 4 atypical genotypes. Ninety percent (103 of 115) of isolates were clonal, i.e., Type II (n = 61) and Type III (n = 42) strains. Of the 61 Type II strains, all had the Type II alleles at all loci, except for 2 strains that had allele I at Apico. Eight isolates were divided into 4 atypical genotypes. One of these genotypes (with 4 isolates) was previously reported in dogs from Sri Lanka and in sand cats from the United Arab Emirates. Four isolates had mixed infections. These results revealed a strong clonal population structure with the dominance of clonal Type II and III lineages of T. gondii in feral cats from Egypt.
猫在刚地弓形虫的流行病学中很重要，因为它们是唯一会在粪便中排出具有环境抵抗力的卵囊的宿主。在本研究中，使用10种聚合酶链反应-限制性片段长度多态性标记（SAG1、SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1和Apico）以及速殖子的DNA，对来自埃及猫组织的115株活的刚地弓形虫分离株进行了基因分型。识别出了7种基因型，包括克隆性II型、III型（2种基因型）和4种非典型基因型。90%（115株中的103株）的分离株是克隆性的，即II型（n = 61）和III型（n = 42）菌株。在61株II型菌株中，除了2株在Apico位点具有等位基因I外，所有菌株在所有位点都具有II型等位基因。8株分离株被分为4种非典型基因型。其中一种基因型（有4株分离株）先前在斯里兰卡的狗和阿拉伯联合酋长国的沙猫中被报道过。4株分离株有混合感染。这些结果揭示了埃及野猫中刚地弓形虫具有强大的克隆群体结构，克隆性II型和III型谱系占主导地位。
