Azizi Hakim, Hataminejad Maryam, Taghipour Ali, Norouzi Maryam, Mirzapour Aliyar
Department of Medical Parasitology, Zabol University of Medical Sciences, Zabol, Iran.
Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran.
Vet Anim Sci. 2024 Aug 15;25:100389. doi: 10.1016/j.vas.2024.100389. eCollection 2024 Sep.
Cats, being the definitive host of , have a significant impact on the spread and outbreaks of the parasite. An essential factor in comprehending the transmission pattern of this parasite is an analysis of the genetic diversity distribution in cats infected with . This study was aimed at determining the prevalence rate and genotyping of in stray cat feces from Khorramabad, West Iran. In the years 2016-2017, 200 cats were sampled to get fresh feces specimens. Parasitological methods were utilized for the identification of oocysts. The DNA was isolated from the feces using a commercially available Genomic Mini Kit. In order to identify the genetic composition of , we employed PCR-RFLP, sequencing, and phylogenetic analysis of the GRA6 target gene. No one of the samples tested positive for parasitology techniques. A total of 6.5 % (13/200) samples were positive when using the GRA6-PCR method. Based on PCR-RFLP results, all 13 samples were of type III genotype. The nucleotide sequences of two samples from this study were found to be 5 % different from those of 12 references of and one strain of that was used as an external control. Based on the findings, molecular tests are more sensitive than parasitological methods. The RFLP approach revealed that type III of is the prevailing and important genotype in Khorramabad, West Iran.
猫作为[寄生虫名称]的终末宿主，对该寄生虫的传播和暴发具有重大影响。理解这种寄生虫传播模式的一个关键因素是分析感染[寄生虫名称]的猫的遗传多样性分布情况。本研究旨在确定伊朗西部霍拉马巴德流浪猫粪便中[寄生虫名称]的流行率和基因分型。在2016 - 2017年期间，采集了200只猫的新鲜粪便样本。采用寄生虫学方法鉴定卵囊。使用市售的基因组迷你试剂盒从粪便中分离DNA。为了鉴定[寄生虫名称]的基因组成，我们对GRA6靶基因进行了PCR - RFLP、测序和系统发育分析。寄生虫学检测方法的所有样本均为阴性。使用GRA6 - PCR方法时，共有6.5%（13/200）的样本呈阳性。基于PCR - RFLP结果，所有13个样本均为III型基因型。本研究中两个样本的核苷酸序列与12个[寄生虫名称]参考序列和用作外部对照的一个[寄生虫名称]菌株的序列有5%的差异。基于这些发现，分子检测比寄生虫学方法更敏感。RFLP方法显示，在伊朗西部霍拉马巴德，[寄生虫名称]的III型是主要且重要的基因型。
