Yang Yurong, Dong Hui, Su Ruijing, Li Tongyi, Jiang Nan, Su Chunlei, Zhang Longxian
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR China.
Zhengzhou Zoo, Zhengzhou, PR China.
Int J Parasitol Parasites Wildl. 2019 Feb 23;8:188-191. doi: 10.1016/j.ijppaw.2019.02.006. eCollection 2019 Apr.
has been found to infect almost all warm-blooded animals; however, some hosts lack direct evidence of infection. The red panda () is an endangered species that mainly lives in temperate forests of South Asia. Here, infection in red pandas from zoos in China were reported. Antibodies to were found in 14.3% (2/14) of red pandas via the modified agglutination test (MAT) with a cut-off titer of 1:25. One viable strain was isolated from tissues of red panda and designated as TgRedpandaCHn1. DNA from tachyzoites obtained from cell culture was characterized by PCR-RFLP with 10 markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) and virulence genes of ROP5 and ROP18. The results indicate that this isolate belonged to ToxoDB genotype #20. The ROP18/ROP5 genotype combination predicated that this strain is non-lethal to mice, which is supported by the infection in mice. tissue cysts were readily formed and mice survived. Tissue cysts observed in the histopathological sections of the tongue and diaphragm of one red panda were speculated as sarcocysts, but not base on morphological characteristics. To our knowledge, this study is the first to report on the isolation of from red panda. Additionally, this report provides direct evidence of red panda as an intermediate host of and species.
已发现其可感染几乎所有温血动物；然而，一些宿主缺乏感染的直接证据。小熊猫（Ailurus fulgens）是一种濒危物种，主要生活在南亚的温带森林中。在此，报道了中国动物园中小熊猫的弓形虫感染情况。通过改良凝集试验（MAT），以1:25的截断滴度，在14.3%（2/14）的小熊猫中发现了弓形虫抗体。从小熊猫组织中分离出一株活的弓形虫菌株，并命名为TgRedpandaCHn1。用10种标记物（SAG1、SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1和Apico）以及ROP5和ROP18的毒力基因，通过PCR-RFLP对细胞培养获得的速殖子DNA进行了特征分析。结果表明，该分离株属于ToxoDB基因型#20。ROP18/ROP5基因型组合预测该菌株对小鼠无致死性，这一点在小鼠感染实验中得到了证实。组织囊肿很容易形成，小鼠存活下来。在一只小熊猫舌头和横膈膜的组织病理学切片中观察到的组织囊肿被推测为肉孢子虫囊肿，但根据形态特征判断并非弓形虫囊肿。据我们所知，本研究首次报道了从小熊猫中分离出弓形虫。此外，本报告提供了小熊猫作为弓形虫中间宿主的直接证据。
