Department of Obstetrics and Gynecology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Reprod Sci. 2011 Jun;18(6):511-5. doi: 10.1177/1933719110391279. Epub 2010 Dec 15.
To investigate the expression of capsaicin receptor (transient receptor potential vanilloid type 1 [TRPV1]) in the peritoneal endometriosis foci of women with and without chronic pelvic pain (CPP).
A case-control study was conducted on 49 women with endometriosis who underwent laparoscopy, 28 of whom had CPP and 21 without CPP. Samples from peritoneum of the rectouterine excavation (2 cm2) were obtained by laparoscopy, fixed in 4% formaldehyde, and underwent immunohistochemistry analysis using rabbit anti-TRPV1 (1:400) polyclonal antibody.
Image analysis revealed that the immunoreactivity for TRPV1 was more frequent in specimens (endometriosis foci) from women with CPP (n = 15 of 28, 53.6%), compared to samples from the endometriosis foci of women without CPP (n = 6 of 21, 28.6%; P = .04). There was no correlation with duration, intensity of pain, or stage of the disease (endometriosis).
The present study shows that TRPV1 expression in peritoneal endometriosis foci is related to CPP in women. However, this association is not related to the endometriosis stage. In view of the immunoreactivity for TRPV1 observed here, we believe that some endometriotic lesions may provide a scenario for TRPV1 to be tonically active and this activity may contribute to the underlying pathology of CPP.
研究辣椒素受体（瞬时受体电位香草酸型 1 [TRPV1]）在有和无慢性盆腔疼痛（CPP）的女性腹膜子宫内膜异位症病灶中的表达。
对 49 例行腹腔镜检查的子宫内膜异位症女性进行病例对照研究，其中 28 例有 CPP，21 例无 CPP。通过腹腔镜获取直肠子宫陷凹（2 cm2）腹膜样本，用 4%甲醛固定，并使用兔抗 TRPV1（1:400）多克隆抗体进行免疫组织化学分析。
图像分析显示，有 CPP 的女性（28 例中的 15 例，53.6%）标本（子宫内膜异位症病灶）中 TRPV1 的免疫反应性较无 CPP 的女性（21 例中的 6 例，28.6%；P =.04）更频繁。与疼痛持续时间、强度或疾病（子宫内膜异位症）阶段均无相关性。
本研究表明，腹膜子宫内膜异位症病灶中 TRPV1 的表达与女性 CPP 相关。然而，这种相关性与子宫内膜异位症的分期无关。鉴于此处观察到的 TRPV1 免疫反应性，我们认为一些子宫内膜异位病变可能为 TRPV1 持续活跃提供了一种情况，这种活性可能有助于 CPP 的潜在病理学。
