Chamovitz D, Pecker I, Sandmann G, Böger P, Hirschberg J
Department of Genetics, Hebrew University of Jerusalem, Israel.
Z Naturforsch C J Biosci. 1990 May;45(5):482-6. doi: 10.1515/znc-1990-0531.
The herbicide norflurazon inhibits carotene biosynthesis in photosynthetic organisms by blocking the enzyme phytoene dehydrogenase (= phytoene desaturase). We have isolated norflurazon-resistant mutants of the cyanobacterium Synechococcus PCC7942. The herbicide-resistance gene from the mutant NFZ4 has been cloned by genetic complementation of the resistance trait in wild type cells. The experiment described here illustrates the usefulness of employing cyanobacteria to clone herbicide-resistance genes in a quick and simple way.
除草剂去草胺通过抑制八氢番茄红素脱氢酶（= 八氢番茄红素去饱和酶）来抑制光合生物中的类胡萝卜素生物合成。我们已经分离出了集胞藻PCC7942的去草胺抗性突变体。通过在野生型细胞中对抗性性状进行遗传互补，克隆了突变体NFZ4中的除草剂抗性基因。此处描述的实验说明了利用蓝细菌以快速简便的方式克隆除草剂抗性基因的实用性。
