通过种间接合和染色体重组在聚球藻属菌株PCC 7942和PCC 6301中进行高效基因转移。
Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination.
作者信息
Tsinoremas N F, Kutach A K, Strayer C A, Golden S S
机构信息
Department of Biology, Texas A&M University, College Station 77843-3258.
出版信息
J Bacteriol. 1994 Nov;176(21):6764-8. doi: 10.1128/jb.176.21.6764-6768.1994.
Abstract
We developed a versatile, efficient genetic transfer method for Synechococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transformation efficiencies by orders of magnitude. As a test case, we complemented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementing gene.
摘要
我们为聚球藻属菌株PCC 7942和PCC 6301开发了一种通用、高效的基因转移方法，其转化效率比自然转化效率高出几个数量级。作为一个测试案例，我们对一个组氨酸营养缺陷型进行了互补，并鉴定出PCC 7942的一个hisS同源基因作为互补基因。
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