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Isolation and Characterization of High CO(2)-Requiring-Mutants of the Cyanobacterium Synechococcus PCC7942 : Two Phenotypes that Accumulate Inorganic Carbon but Are Apparently Unable to Generate CO(2) within the Carboxysome.集胞藻PCC7942中高二氧化碳需求突变体的分离与鉴定:两种积累无机碳但显然无法在羧酶体中产生二氧化碳的表型。
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通过种间接合和染色体重组在聚球藻属菌株PCC 7942和PCC 6301中进行高效基因转移。

Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination.

作者信息

Tsinoremas N F, Kutach A K, Strayer C A, Golden S S

机构信息

Department of Biology, Texas A&M University, College Station 77843-3258.

出版信息

J Bacteriol. 1994 Nov;176(21):6764-8. doi: 10.1128/jb.176.21.6764-6768.1994.

DOI:10.1128/jb.176.21.6764-6768.1994
PMID:7961432
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC197036/
Abstract

We developed a versatile, efficient genetic transfer method for Synechococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transformation efficiencies by orders of magnitude. As a test case, we complemented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementing gene.

摘要

我们为聚球藻属菌株PCC 7942和PCC 6301开发了一种通用、高效的基因转移方法,其转化效率比自然转化效率高出几个数量级。作为一个测试案例,我们对一个组氨酸营养缺陷型进行了互补,并鉴定出PCC 7942的一个hisS同源基因作为互补基因。