Department of School of Management and Science, West Bengal University of Technology, Salt lake, Kolkata, 700064, India.
Glycoconj J. 2011 Jan;28(1):1-9. doi: 10.1007/s10719-010-9316-z. Epub 2010 Dec 14.
The level of serum glycoproteins and their glycosylation pattern change in liver diseases including hepatocellular carcinoma (HCC). Some of them, especially alpha fetoprotein (AFP), serve as useful biomarkers for HCC. The present investigation showed high level of AFP in hepatitis B cirrhosis (HBV-LC) and hepatitis C cirrhosis (HCV-LC) patients. However, increase of AFP level was not significantly high in chronic hepatitis B (HBV-CH) as determined by ELISA using monoclonal anti-human AFP (mAb-AFP). The differential expression of sialic acid linkage was observed in HBV-CH and HCV-LC by ELISA; the former bound strongly with Sambucus nigra agglutinin (SNA), which has exclusive binding specificity for NeuAcα2-6-, whereas HCV-LC reacted preferably with Maackia amurensis agglutinin (MAA) which recognizes NeuAcα2-3-. There was significantly high glycan branching in HBV-LC and HCV-LC in comparison to controls as illustrated by concanavalin A. This was further confirmed by Phaseolus vulgaris erythroagglutinin (E-PHA) and Datura stramonium agglutinin (DSA). Enhanced fucosylation of AFP was observed in HBV-LC, HCV-LC and HCC patients by ELISA using fucose binding Aleuria aurantia lectin; however, maximum binding was found in HCC. Fucosylation with α1-6 linkage was further confirmed by Lens culinaris agglutinin (LCA). From the above results it is concluded that the changes in concentration of AFP, differential expression of sialic acid, increase of glycan branching and fucosylation have a prognostic value of hepatitis and it could be possible that lectin-based assay with AFP can aid in diagnosis of hepatitis diseases besides clinical examination and routine laboratory investigation.
血清糖蛋白及其糖基化模式在包括肝细胞癌（HCC）在内的肝脏疾病中发生变化。其中一些，特别是甲胎蛋白（AFP），可作为 HCC 的有用生物标志物。本研究表明乙型肝炎肝硬化（HBV-LC）和丙型肝炎肝硬化（HCV-LC）患者的 AFP 水平较高。然而，ELISA 法使用单克隆抗人 AFP（mAb-AFP）检测发现，乙型肝炎慢性期（HBV-CH）的 AFP 水平升高不明显。通过 ELISA 观察到 HBV-CH 和 HCV-LC 中唾液酸连接的差异表达；前者与具有独特 NeuAcα2-6-结合特异性的 Sambucus nigra 凝集素（SNA）强烈结合，而 HCV-LC 与识别 NeuAcα2-3-的 Maackia amurensis 凝集素（MAA）反应较好。与对照组相比，HBV-LC 和 HCV-LC 中的聚糖分支明显较高，如 Concanavalin A 所示。用 Phaseolus vulgaris erythroagglutinin（E-PHA）和 Datura stramonium agglutinin（DSA）进一步证实了这一点。用结合岩藻糖的 Aleuria aurantia 凝集素（AFU）通过 ELISA 观察到 HBV-LC、HCV-LC 和 HCC 患者 AFP 的岩藻糖基化增强；然而，在 HCC 中发现最大结合。用 Lens culinaris 凝集素（LCA）进一步证实了与α1-6 键的岩藻糖基化。从上述结果得出结论，AFP 浓度的变化、唾液酸的差异表达、聚糖分支和岩藻糖基化的增加对肝炎具有预后价值，并且基于凝集素的 AFP 测定除了临床检查和常规实验室检查外，还可能有助于肝炎疾病的诊断。
