Department of Natural Science, Humanities and Management, West Bengal University of Technology, BF-142, Salt Lake, Sector-1, Kolkata, 700 064, India.
Glycoconj J. 2009 Dec;26(9):1225-34. doi: 10.1007/s10719-009-9241-1.
Altered glycosylation and concentration of alpha1-acid glycoprotein has been known to be related to the pathogenesis of the hepatic diseases. The present study investigated enhanced fucosylation of AGP in the sera of chronic hepatitis B (HBV-CH) and hepatitis B cirrhosis (HBV-LC) patients by high performance anion exchange chromatography and by ELISA using fucose binding Aleuria aurantia lectin. The concentration of AGP determined by ELISA using monoclonal anti-human AGP (mAb-AGP) showed high level of AGP in HBV-CH and HBV-LC patients. This was further judged by association constant (K (A)) measured by surface plasmon resonance analysis. There was no apparent linkage variation of sialic acid among different patient groups when tested with two sialic acid binding lectins viz., Maackia amurensis agglutinin (MAA, NeuAc alpha2-3-) and Sambucus nigra agglutinin (SNA, NeuAc alpha2-6-) respectively. There was no change of oligosaccharide branching in HBV-CH in comparison to controls whereas a slight change was observed in HBV-LC using ConA. The above results suggest that the changes in concentration of AGP and fucosylation have a prognostic value of hepatitis diseases and it could be possible to use AGP as diagnostic marker besides clinical examination and routine laboratory investigation.
已知糖基化和α1-酸性糖蛋白的浓度与肝脏疾病的发病机制有关。本研究通过高效阴离子交换色谱法和使用结合岩藻糖的Aleuria aurantia 凝集素的 ELISA 法，研究了慢性乙型肝炎 (HBV-CH) 和乙型肝炎肝硬化 (HBV-LC) 患者血清中 AGP 的增强岩藻糖化。使用单克隆抗人 AGP (mAb-AGP) 的 ELISA 法测定的 AGP 浓度显示 HBV-CH 和 HBV-LC 患者的 AGP 水平较高。通过表面等离子体共振分析测量的关联常数 (K(A)) 进一步判断。当使用两种唾液酸结合凝集素即 Maackia amurensis agglutinin (MAA，NeuAc alpha2-3-) 和 Sambucus nigra agglutinin (SNA，NeuAc alpha2-6-) 测试时，不同患者组之间的唾液酸连接变异没有明显差异。与对照相比，HBV-CH 中的寡糖分支没有变化，而在用 ConA 时，HBV-LC 中观察到轻微变化。上述结果表明，AGP 浓度和岩藻糖化的变化对肝炎疾病具有预后价值，除了临床检查和常规实验室检查外，AGP 可能可用作诊断标志物。
