Key Lab of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China.
Mol Biol Rep. 2011 Nov;38(8):4999-5002. doi: 10.1007/s11033-010-0645-6. Epub 2010 Dec 14.
The mud crab Scylla paramamosain plays a significant role in fishery resources in China. In this study, we developed 18 polymorphic microsatellite markers in this important crab by 5' anchored PCR technique. A total of 125 alleles were detected in a single population of 32 individuals of S. paramamosain. The number of alleles per locus ranged from five to nine, with the allele size ranging from 166 to 316 bp. The polymorphism information content (PIC), observed and expected heterozygosity ranged from 0.39 to 0.88, from 0.33 to 0.92 and from 0.42 to 0.86, respectively. Three loci (Scypa13, Scypa14 and Scypa15) deviated significantly from Hardy-Weinberg equilibrium (HWE) after Bonferroni correction (P < 0.0028), and no linkage disequilibrium was found between loci pairs. These polymorphic microsatellite markers will be useful for the study of population genetic structure, construction of genetic linkage maps and mapping of economically quantitative trait loci (QTL) in S. paramamosain.
拟穴青蟹在我国渔业资源中占有重要地位。本研究采用 5'锚定 PCR 技术，从拟穴青蟹基因组中开发出 18 对多态性微卫星标记。在对 32 个单一个体的检测中，共发现 125 个等位基因，每个位点的等位基因数为 5-9 个，大小为 166-316 bp。多态信息含量(PIC)、观测杂合度和期望杂合度分别为 0.39-0.88、0.33-0.92 和 0.42-0.86。经过 Bonferroni 校正后，有 3 个位点（Scypa13、Scypa14 和 Scypa15）偏离 Hardy-Weinberg 平衡（HWE）（P<0.0028），并且未发现位点间的连锁不平衡。这些多态性微卫星标记将有助于研究拟穴青蟹的群体遗传结构、构建遗传连锁图谱和定位经济数量性状基因座（QTL）。
