Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Mol Biol Rep. 2011 Oct;38(7):4469-74. doi: 10.1007/s11033-010-0576-2. Epub 2010 Dec 15.
Four groups of 12 young beef, as similar as possible with respect to age and weight, were fed a basic diet. The addition fed to four groups was 0, 200, 600, and 1,200 mg of organic chromium (chromium picolinate CrPic) per kg concentrated feed. The results showed that there was no effect on overall growth performance and dressing percentage and pure meat percentage when adding different CrPic content, but significant difference was found between 0 group and other three groups in percentage of high grade cuts (P < 0.05). The Cr content in Kidney, Musculus diaphragm, Semitendinosus muscles and Longissimusdorsi tissues have no difference in four groups (P > 0.05), but there was significant difference in liver tissues between 0, 200, 600 groups and 1200 group (P < 0.05). Gene expression analysis indicated that there were no differences in five genes expression in liver and muscle tissues in four groups (P > 0.05), but mRNA expression amount of FOX1, FSTL and MATR3 always had same trends whatever in liver or muscle tissues. However the RPLOP gene expression amount has large difference between liver and muscle.
四组 12 头年轻的肉牛，在年龄和体重方面尽可能相似，均喂食基础日粮。另外四组分别添加 0、200、600 和 1200mg/kg 浓缩饲料的有机铬（吡啶甲酸铬 CrPic）。结果表明，添加不同含量的 CrPic 对整体生长性能和屠宰率、净肉率没有影响，但 0 组与其他三组在高档切块率方面存在显著差异（P<0.05）。四组牛肾、膈肌、半腱肌和背最长肌组织中的铬含量无差异（P>0.05），但 0、200、600 组与 1200 组肝脏组织中的铬含量存在显著差异（P<0.05）。基因表达分析表明，四组牛肝脏和肌肉组织中 5 个基因的表达无差异（P>0.05），但 FOX1、FSTL 和 MATR3 的 mRNA 表达量在肝脏或肌肉组织中始终呈相同趋势。然而，RPLOP 基因在肝脏和肌肉中的表达量差异较大。
